Generating antibodies to the gene 3 proteins of infectious bronchitis virus

Amanda R. Pendleton, Carolyn E. MacHamer

Research output: Chapter in Book/Report/Conference proceedingChapter


Infectious bronchitis virus (IBV), a group 3 coronavirus, produces three proteins (IBV E, IBV 3a, and IBV 3b) from subgenomic mRNA 3 during infection. IBV E, a viral envelope protein, plays a role in virus budding, possibly by altering membrane morphology at the virus assembly site. In addition to this role, IBV E may also function as a viroporin, although no data from infected cells have confirmed this possibility definitively. Conversely, the IBV 3a and IBV 3b proteins are nonstructural proteins. These proteins are dispensable for replication in cell culture, but are thought to be important for infection of the natural host. This chapter details methods for generating and screening antibodies to these gene 3 proteins. Antibodies were raised in rabbits following inoculation with IBV-specific peptides and GST fusion proteins, and were screened by immunofluorescence, radioimmunoprecipitation, and immunoblotting.

Original languageEnglish (US)
Title of host publicationSARS- and Other Coronaviruses
Subtitle of host publicationLaboratory Protocols
PublisherHumana Press
Number of pages27
ISBN (Print)9781588298676
StatePublished - 2008

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745


  • Accessory proteins
  • Antibodies
  • Coronavirus
  • Infectious bronchitis virus, 3a, 3b, E

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics


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