Generating antibodies to the gene 3 proteins of infectious bronchitis virus.

Amanda R. Pendleton, Carolyn E. Machamer

Research output: Contribution to journalArticle

Abstract

Infectious bronchitis virus (IBV), a group 3 coronavirus, produces three proteins (IBV E, IBV 3a, and IBV 3b) from subgenomic mRNA 3 during infection. IBV E, a viral envelope protein, plays a role in virus budding, possibly by altering membrane morphology at the virus assembly site. In addition to this role, IBV E may also function as a viroporin, although no data from infected cells have confirmed this possibility definitively. Conversely, the IBV 3a and IBV 3b proteins are nonstructural proteins. These proteins are dispensable for replication in cell culture, but are thought to be important for infection of the natural host. This chapter details methods for generating and screening antibodies to these gene 3 proteins. Antibodies were raised in rabbits following inoculation with IBV-specific peptides and GST fusion proteins, and were screened by immunofluorescence, radioimmunoprecipitation, and immunoblotting.

Original languageEnglish (US)
Pages (from-to)163-189
Number of pages27
JournalMethods in molecular biology (Clifton, N.J.)
Volume454
StatePublished - 2008

Fingerprint

Infectious bronchitis virus
Antibodies
Proteins
Viral Envelope Proteins
Virus Release
Virus Assembly
Coronavirus
Infection
Immunoblotting
Fluorescent Antibody Technique
Cell Culture Techniques
Rabbits

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Generating antibodies to the gene 3 proteins of infectious bronchitis virus. / Pendleton, Amanda R.; Machamer, Carolyn E.

In: Methods in molecular biology (Clifton, N.J.), Vol. 454, 2008, p. 163-189.

Research output: Contribution to journalArticle

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