General and Facile Coating of Single Cells via Mild Reduction

Hyunbum Kim, Kwangsoo Shin, Ok Kyu Park, Daheui Choi, Hwan D. Kim, Seungmin Baik, Soo Hong Lee, Seung Hae Kwon, Kevin J. Yarema, Jinkee Hong, Taeghwan Hyeon, Nathaniel S. Hwang

Research output: Contribution to journalArticlepeer-review

Abstract

Cell surface modification has been extensively studied to enhance the efficacy of cell therapy. Still, general accessibility and versatility are remaining challenges to meet the increasing demand for cell-based therapy. Herein, we present a facile and universal cell surface modification method that involves mild reduction of disulfide bonds in cell membrane protein to thiol groups. The reduced cells are successfully coated with biomolecules, polymers, and nanoparticles for an assortment of applications, including rapid cell assembly, in vivo cell monitoring, and localized cell-based drug delivery. No adverse effect on cellular morphology, viability, proliferation, and metabolism is observed. Furthermore, simultaneous coating with polyethylene glycol and dexamethasone-loaded nanoparticles facilitates enhanced cellular activities in mice, overcoming immune rejection.

Original languageEnglish (US)
Pages (from-to)1199-1202
Number of pages4
JournalJournal of the American Chemical Society
Volume140
Issue number4
DOIs
StatePublished - Jan 31 2018

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry

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