TY - JOUR
T1 - General and Facile Coating of Single Cells via Mild Reduction
AU - Kim, Hyunbum
AU - Shin, Kwangsoo
AU - Park, Ok Kyu
AU - Choi, Daheui
AU - Kim, Hwan D.
AU - Baik, Seungmin
AU - Lee, Soo Hong
AU - Kwon, Seung Hae
AU - Yarema, Kevin J.
AU - Hong, Jinkee
AU - Hyeon, Taeghwan
AU - Hwang, Nathaniel S.
N1 - Funding Information:
This work was supported by National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT Grant (NRF-2017M3A9C6029699, NRF-2016R1E1A1A01943393) (N.S.H.) and Institute for Basic Science (IBS) in Korea (IBS-R006-D1) (T.H.).
Publisher Copyright:
© 2017 American Chemical Society.
PY - 2018/1/31
Y1 - 2018/1/31
N2 - Cell surface modification has been extensively studied to enhance the efficacy of cell therapy. Still, general accessibility and versatility are remaining challenges to meet the increasing demand for cell-based therapy. Herein, we present a facile and universal cell surface modification method that involves mild reduction of disulfide bonds in cell membrane protein to thiol groups. The reduced cells are successfully coated with biomolecules, polymers, and nanoparticles for an assortment of applications, including rapid cell assembly, in vivo cell monitoring, and localized cell-based drug delivery. No adverse effect on cellular morphology, viability, proliferation, and metabolism is observed. Furthermore, simultaneous coating with polyethylene glycol and dexamethasone-loaded nanoparticles facilitates enhanced cellular activities in mice, overcoming immune rejection.
AB - Cell surface modification has been extensively studied to enhance the efficacy of cell therapy. Still, general accessibility and versatility are remaining challenges to meet the increasing demand for cell-based therapy. Herein, we present a facile and universal cell surface modification method that involves mild reduction of disulfide bonds in cell membrane protein to thiol groups. The reduced cells are successfully coated with biomolecules, polymers, and nanoparticles for an assortment of applications, including rapid cell assembly, in vivo cell monitoring, and localized cell-based drug delivery. No adverse effect on cellular morphology, viability, proliferation, and metabolism is observed. Furthermore, simultaneous coating with polyethylene glycol and dexamethasone-loaded nanoparticles facilitates enhanced cellular activities in mice, overcoming immune rejection.
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U2 - 10.1021/jacs.7b08440
DO - 10.1021/jacs.7b08440
M3 - Article
C2 - 29281277
AN - SCOPUS:85041332656
SN - 0002-7863
VL - 140
SP - 1199
EP - 1202
JO - Journal of the American Chemical Society
JF - Journal of the American Chemical Society
IS - 4
ER -