Gene transfer in vivo with DNA-liposome complexes: Safety and acute toxicity in mice

Mark J. Stewart, Gregory E. Plautz, Lisa Del Buono, Zhi Yong Yang, Ling Xu, Xiang Gao, Leaf Huang, Elizabeth G. Nabel, Gary J. Nabel

Research output: Contribution to journalArticlepeer-review

Abstract

DNA can be introduced into a variety of cell types after formation of liposomal complexes with cationic lipids. In this report, conditions have been established to optimize the production of DNA-liposome complexes that efficiently transfect cells. The safety and toxicity of this method of gene delivery have been assessed after in vivo administration, either by intravenous or direct intratumor injection. Nine to eleven days after intravenous injection, DNA was found primarily in heart and lung tissue by PCR analysis. No abnormalities were evident from histologic examination of tissue, examination of tissue-specific serum enzymes, routine biochemical parameters, or electrocardiographic monitoring. DNA-liposome complexes can therefore be used for the delivery of recombinant genes in vivo with minimal toxicity.

Original languageEnglish (US)
Pages (from-to)267-275
Number of pages9
JournalHuman Gene Therapy
Volume3
Issue number3
StatePublished - 1992
Externally publishedYes

ASJC Scopus subject areas

  • Genetics

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