Gene transfection efficacy assessment of human cervical cancer cells using dual-mode fluorescence microendoscopy

Jaepyeong Cha; Jing Zhang; Saumya Gurbani; Gyeong Woo Cheon; Min Li; Jin U. Kang

doi:10.1364/BOE.4.000151

Gene transfection efficacy assessment of human cervical cancer cells using dual-mode fluorescence microendoscopy

Jaepyeong Cha, Jing Zhang, Saumya Gurbani, Gyeong Woo Cheon, Min Li, Jin U. Kang

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

We report a novel approach to quantitatively assess gene transfection efficacy using dual-modality microendoscopy that can simultaneously monitor both laser scanning reflectance and fluorescence imaging. The system uses a 500-μm-diameter coherent fiber bundle and permits 3.5-μm lateral resolution. Both reflectance and fluorescence images obtained from two silicon avalanche photodetectors are displaying at 1 Hz and processed automatically to calculate gene transfection efficiency (the ratio of fluorescent cells among the total cells). To validate the system performance we examined the expression of cyan fluorescent protein using human cervical cancer cells (HeLa) in four commercially available reagents. The result was compared with that using a high-resolution bench-top microscope.

Original language	English (US)
Pages (from-to)	151-159
Number of pages	9
Journal	Biomedical Optics Express
Volume	4
Issue number	1
DOIs	https://doi.org/10.1364/BOE.4.000151
State	Published - 2013
Externally published	Yes

ASJC Scopus subject areas

Biotechnology
Atomic and Molecular Physics, and Optics

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abstract = "We report a novel approach to quantitatively assess gene transfection efficacy using dual-modality microendoscopy that can simultaneously monitor both laser scanning reflectance and fluorescence imaging. The system uses a 500-μm-diameter coherent fiber bundle and permits 3.5-μm lateral resolution. Both reflectance and fluorescence images obtained from two silicon avalanche photodetectors are displaying at 1 Hz and processed automatically to calculate gene transfection efficiency (the ratio of fluorescent cells among the total cells). To validate the system performance we examined the expression of cyan fluorescent protein using human cervical cancer cells (HeLa) in four commercially available reagents. The result was compared with that using a high-resolution bench-top microscope.",

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AU - Li, Min

AU - Kang, Jin U.

PY - 2013

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AB - We report a novel approach to quantitatively assess gene transfection efficacy using dual-modality microendoscopy that can simultaneously monitor both laser scanning reflectance and fluorescence imaging. The system uses a 500-μm-diameter coherent fiber bundle and permits 3.5-μm lateral resolution. Both reflectance and fluorescence images obtained from two silicon avalanche photodetectors are displaying at 1 Hz and processed automatically to calculate gene transfection efficiency (the ratio of fluorescent cells among the total cells). To validate the system performance we examined the expression of cyan fluorescent protein using human cervical cancer cells (HeLa) in four commercially available reagents. The result was compared with that using a high-resolution bench-top microscope.

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Gene transfection efficacy assessment of human cervical cancer cells using dual-mode fluorescence microendoscopy

Abstract

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