Gene expression signatures of primary and metastatic uterine leiomyosarcoma

Ben Davidson, Vera Maria Abeler, Mette Førsund, Arild Holth, Yanqin Yang, Yusuke Kobayashi, Lily Chen, Gunnar B. Kristensen, Ie Ming Shih, Tian-Li Wang

Research output: Contribution to journalArticle

Abstract

Leiomyosarcoma (LMS) is the most common uterine sarcoma. Although the disease is relatively rare, it is responsible for considerable mortality due to frequent metastasis and chemoresistance. The molecular events related to LMS metastasis are unknown to date. The present study compared the global gene expression patterns of primary uterine LMSs and LMS metastases. Gene expression profiles of 13 primary and 15 metastatic uterine LMSs were analyzed using the HumanRef-8 BeadChip from Illumina. Differentially expressed candidate genes were validated using quantitative real-time polymerase chain reaction (PCR) and immunohistochemistry. To identify differently expressed genes between primary and metastatic tumors, we performed one-way analysis of variance with Benjamini-Hochberg correction. This led to identification of 203 unique probes that were significantly differentially expressed in the 2 tumor groups by greater than 1.58-fold with P

Original languageEnglish (US)
Pages (from-to)691-700
Number of pages10
JournalHuman Pathology
Volume45
Issue number4
DOIs
StatePublished - 2014

Fingerprint

Leiomyosarcoma
Transcriptome
Neoplasm Metastasis
Sarcoma
Genes
Real-Time Polymerase Chain Reaction
Neoplasms
Analysis of Variance
Immunohistochemistry
Gene Expression
Mortality

Keywords

  • Gene expression array
  • Metastasis
  • Uterine leiomyosarcoma

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Davidson, B., Abeler, V. M., Førsund, M., Holth, A., Yang, Y., Kobayashi, Y., ... Wang, T-L. (2014). Gene expression signatures of primary and metastatic uterine leiomyosarcoma. Human Pathology, 45(4), 691-700. https://doi.org/10.1016/j.humpath.2013.11.003

Gene expression signatures of primary and metastatic uterine leiomyosarcoma. / Davidson, Ben; Abeler, Vera Maria; Førsund, Mette; Holth, Arild; Yang, Yanqin; Kobayashi, Yusuke; Chen, Lily; Kristensen, Gunnar B.; Shih, Ie Ming; Wang, Tian-Li.

In: Human Pathology, Vol. 45, No. 4, 2014, p. 691-700.

Research output: Contribution to journalArticle

Davidson, B, Abeler, VM, Førsund, M, Holth, A, Yang, Y, Kobayashi, Y, Chen, L, Kristensen, GB, Shih, IM & Wang, T-L 2014, 'Gene expression signatures of primary and metastatic uterine leiomyosarcoma', Human Pathology, vol. 45, no. 4, pp. 691-700. https://doi.org/10.1016/j.humpath.2013.11.003
Davidson B, Abeler VM, Førsund M, Holth A, Yang Y, Kobayashi Y et al. Gene expression signatures of primary and metastatic uterine leiomyosarcoma. Human Pathology. 2014;45(4):691-700. https://doi.org/10.1016/j.humpath.2013.11.003
Davidson, Ben ; Abeler, Vera Maria ; Førsund, Mette ; Holth, Arild ; Yang, Yanqin ; Kobayashi, Yusuke ; Chen, Lily ; Kristensen, Gunnar B. ; Shih, Ie Ming ; Wang, Tian-Li. / Gene expression signatures of primary and metastatic uterine leiomyosarcoma. In: Human Pathology. 2014 ; Vol. 45, No. 4. pp. 691-700.
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