Gene expression profiling of diffuse large B-cell lymphoma supervised by CD21 expression

Kana Miyazaki, Motoko Yamaguchi, Miyuki Suguro, Woonyoung Choi, Yuan Ji, Lianchun Xiao, Wei Zhang, Shoko Ogawa, Naoyuki Katayama, Hiroshi Shiku, Tohru Kobayashi

Research output: Contribution to journalArticle

Abstract

This study investigated the gene expression profiles of 40 cases of diffuse large B-cell lymphoma (DLBCL) according to CD21 expression, a favourable prognostic factor in DLBCL. Signature genes were analysed by Gene Ontology Tree Machine, and genes concerned with the immune system and related categories were significantly upregulated in CD21- DLBCLs. Of 40 DLBCLs, four were germinal centre B cell-like (GCB) and 36 non-GCB. Of the 36 non-GCB DLBCLs, 14 CD21+ DLBCLs showed significantly better overall survival than the 22 CD21- DLBCLs (P = 0.036). Hierarchical cluster analysis of signature genes related to CD21 was applied to previously published data sets, resulting in two groups for each data set, CD21+ type DLBCLs and CD21 - type DLBCLs. Survival of CD21+ type DLBCLs was significantly better than that of CD21- type (P = 0.006 and P = 0.004, respectively). In both data sets, CD21+ type DLBCLs predominantly included GCB DLBCLs compared with CD21- type. The top classifier gene of CD21 expression was IGHM, and the five of nine Gene Ontology categories significant in CD21- DLBCLs included IGHM. Immunohistochemical analysis of 216 DLBCLs confirmed that overall survival of surface (s) IgM+ DLBCLs was significantly poorer than that of sIgM- DLBCLs (P = 0.013).

Original languageEnglish (US)
Pages (from-to)562-570
Number of pages9
JournalBritish Journal of Haematology
Volume142
Issue number4
DOIs
StatePublished - Aug 1 2008
Externally publishedYes

Fingerprint

Lymphoma, Large B-Cell, Diffuse
Gene Expression Profiling
Gene Ontology
Genes
Germinal Center
Transcriptome
Immunoglobulin M
Cluster Analysis
Immune System
B-Lymphocytes
Gene Expression
Datasets

Keywords

  • CD21
  • Diffuse large B-cell lymphoma
  • Gene expression profiling
  • Gene Ontology
  • Immunoglobulin M

ASJC Scopus subject areas

  • Hematology

Cite this

Gene expression profiling of diffuse large B-cell lymphoma supervised by CD21 expression. / Miyazaki, Kana; Yamaguchi, Motoko; Suguro, Miyuki; Choi, Woonyoung; Ji, Yuan; Xiao, Lianchun; Zhang, Wei; Ogawa, Shoko; Katayama, Naoyuki; Shiku, Hiroshi; Kobayashi, Tohru.

In: British Journal of Haematology, Vol. 142, No. 4, 01.08.2008, p. 562-570.

Research output: Contribution to journalArticle

Miyazaki, K, Yamaguchi, M, Suguro, M, Choi, W, Ji, Y, Xiao, L, Zhang, W, Ogawa, S, Katayama, N, Shiku, H & Kobayashi, T 2008, 'Gene expression profiling of diffuse large B-cell lymphoma supervised by CD21 expression', British Journal of Haematology, vol. 142, no. 4, pp. 562-570. https://doi.org/10.1111/j.1365-2141.2008.07218.x
Miyazaki, Kana ; Yamaguchi, Motoko ; Suguro, Miyuki ; Choi, Woonyoung ; Ji, Yuan ; Xiao, Lianchun ; Zhang, Wei ; Ogawa, Shoko ; Katayama, Naoyuki ; Shiku, Hiroshi ; Kobayashi, Tohru. / Gene expression profiling of diffuse large B-cell lymphoma supervised by CD21 expression. In: British Journal of Haematology. 2008 ; Vol. 142, No. 4. pp. 562-570.
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AU - Ji, Yuan

AU - Xiao, Lianchun

AU - Zhang, Wei

AU - Ogawa, Shoko

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AU - Shiku, Hiroshi

AU - Kobayashi, Tohru

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N2 - This study investigated the gene expression profiles of 40 cases of diffuse large B-cell lymphoma (DLBCL) according to CD21 expression, a favourable prognostic factor in DLBCL. Signature genes were analysed by Gene Ontology Tree Machine, and genes concerned with the immune system and related categories were significantly upregulated in CD21- DLBCLs. Of 40 DLBCLs, four were germinal centre B cell-like (GCB) and 36 non-GCB. Of the 36 non-GCB DLBCLs, 14 CD21+ DLBCLs showed significantly better overall survival than the 22 CD21- DLBCLs (P = 0.036). Hierarchical cluster analysis of signature genes related to CD21 was applied to previously published data sets, resulting in two groups for each data set, CD21+ type DLBCLs and CD21 - type DLBCLs. Survival of CD21+ type DLBCLs was significantly better than that of CD21- type (P = 0.006 and P = 0.004, respectively). In both data sets, CD21+ type DLBCLs predominantly included GCB DLBCLs compared with CD21- type. The top classifier gene of CD21 expression was IGHM, and the five of nine Gene Ontology categories significant in CD21- DLBCLs included IGHM. Immunohistochemical analysis of 216 DLBCLs confirmed that overall survival of surface (s) IgM+ DLBCLs was significantly poorer than that of sIgM- DLBCLs (P = 0.013).

AB - This study investigated the gene expression profiles of 40 cases of diffuse large B-cell lymphoma (DLBCL) according to CD21 expression, a favourable prognostic factor in DLBCL. Signature genes were analysed by Gene Ontology Tree Machine, and genes concerned with the immune system and related categories were significantly upregulated in CD21- DLBCLs. Of 40 DLBCLs, four were germinal centre B cell-like (GCB) and 36 non-GCB. Of the 36 non-GCB DLBCLs, 14 CD21+ DLBCLs showed significantly better overall survival than the 22 CD21- DLBCLs (P = 0.036). Hierarchical cluster analysis of signature genes related to CD21 was applied to previously published data sets, resulting in two groups for each data set, CD21+ type DLBCLs and CD21 - type DLBCLs. Survival of CD21+ type DLBCLs was significantly better than that of CD21- type (P = 0.006 and P = 0.004, respectively). In both data sets, CD21+ type DLBCLs predominantly included GCB DLBCLs compared with CD21- type. The top classifier gene of CD21 expression was IGHM, and the five of nine Gene Ontology categories significant in CD21- DLBCLs included IGHM. Immunohistochemical analysis of 216 DLBCLs confirmed that overall survival of surface (s) IgM+ DLBCLs was significantly poorer than that of sIgM- DLBCLs (P = 0.013).

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