Gene expression profile in early stage of retinoic acid-induced differentiation of human SH-SY5Y neuroblastoma cells

M. E. Truckenmiller, Marquis P. Vawter, Chris Cheadle, Mark Coggiano, David M. Donovan, William J. Freed, Kevin G. Becker

Research output: Contribution to journalArticle

Abstract

Purpose: The human SH-SY5Y cell line is an established model for retinoic acid (RA)-induced neural differentiation. We employed a broad human 15K microarray (15,000 genes) and focused Neuroarray (1152 genes) to examine changes in gene expression early in the process of differentiation (6 hr), before morphology or growth changes are observed. Methods: 33P-labeled cDNA probes prepared from RNA extracts of RA-treated and control cultures were hybridized to array membranes, and levels of expression were quantified and compared. Results: In the 15K array, 19 % of the genes were decreased (0.4 % were named genes and the remainder were expressed sequence tags (ESTs) or unknowns), and 9 % were increased (4.2 % named genes). In the Neuroarray, 3 % were decreased and 8 % were increased. Conclusions: Summary gene profiles are presented, which include transcription factors, genes associated with cell cycle, cell shape, neurotransmission, intermediary filaments, and others. The prevalence of down-regulated genes in the broad 15K array and up-regulated genes in the neuro-focused array suggests a pattern shift in gene expression associated with differentiation. The predominance of ESTs among the down-regulated genes indicates a great number of as-yet-unidentified genes are repressed in early stage neural differentiation.

Original languageEnglish (US)
Pages (from-to)67-80
Number of pages14
JournalRestorative Neurology and Neuroscience
Volume18
Issue number2-3
StatePublished - 2001
Externally publishedYes

Fingerprint

Tretinoin
Neuroblastoma
Transcriptome
Genes
Expressed Sequence Tags
Gene Expression
Cell Shape
Synaptic Transmission
Cell Cycle
Transcription Factors
Complementary DNA
RNA
Cell Line
Membranes

Keywords

  • Differentiation
  • Microarray
  • Neurofilament
  • Profilin
  • Retinoic acid

ASJC Scopus subject areas

  • Neuroscience(all)
  • Neuropsychology and Physiological Psychology

Cite this

Truckenmiller, M. E., Vawter, M. P., Cheadle, C., Coggiano, M., Donovan, D. M., Freed, W. J., & Becker, K. G. (2001). Gene expression profile in early stage of retinoic acid-induced differentiation of human SH-SY5Y neuroblastoma cells. Restorative Neurology and Neuroscience, 18(2-3), 67-80.

Gene expression profile in early stage of retinoic acid-induced differentiation of human SH-SY5Y neuroblastoma cells. / Truckenmiller, M. E.; Vawter, Marquis P.; Cheadle, Chris; Coggiano, Mark; Donovan, David M.; Freed, William J.; Becker, Kevin G.

In: Restorative Neurology and Neuroscience, Vol. 18, No. 2-3, 2001, p. 67-80.

Research output: Contribution to journalArticle

Truckenmiller, ME, Vawter, MP, Cheadle, C, Coggiano, M, Donovan, DM, Freed, WJ & Becker, KG 2001, 'Gene expression profile in early stage of retinoic acid-induced differentiation of human SH-SY5Y neuroblastoma cells', Restorative Neurology and Neuroscience, vol. 18, no. 2-3, pp. 67-80.
Truckenmiller ME, Vawter MP, Cheadle C, Coggiano M, Donovan DM, Freed WJ et al. Gene expression profile in early stage of retinoic acid-induced differentiation of human SH-SY5Y neuroblastoma cells. Restorative Neurology and Neuroscience. 2001;18(2-3):67-80.
Truckenmiller, M. E. ; Vawter, Marquis P. ; Cheadle, Chris ; Coggiano, Mark ; Donovan, David M. ; Freed, William J. ; Becker, Kevin G. / Gene expression profile in early stage of retinoic acid-induced differentiation of human SH-SY5Y neuroblastoma cells. In: Restorative Neurology and Neuroscience. 2001 ; Vol. 18, No. 2-3. pp. 67-80.
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