Gene expression pathways of high grade localized prostate cancer

Ashley E. Ross, Luigi Marchionni, Milena Vuica-Ross, Chris Cheadle, Jinshui Fan, David M. Berman, Edward M. Schaeffer

Research output: Contribution to journalArticle

Abstract

BACKGROUND Despite advances in screening and local therapy, prostate cancer remains the second most common cause of cancer related death among American men, with those having high grade disease being at highest risk for prostate cancer mortality. Here we identify the genes and cellular pathways that distinguish high grade from low grade pathologically localized prostate cancer. METHODS Cancer cells from low grade (Gleason 3+3=6) or high grade (4+4=8) tumors of men with localized disease were isolated by laser capture micro-dissection. Expression profiling was conducted across 18,344 unique annotated genes and data were analyzed using packages from the R/Bioconductor project to determine differential gene expression and perform gene set enrichment analysis. Publically available expression data was retrieved and analyzed individually in the same manner and in cross platform meta-analyses. RESULTS Six hundred seventy genes were differentially expressed between Gleason sum 6 and 8 tumors with a false discovery rate of

Original languageEnglish (US)
Pages (from-to)1568-1578
Number of pages11
JournalProstate
Volume71
Issue number14
DOIs
Publication statusPublished - Oct 1 2011

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Keywords

  • Gleason score
  • high risk prostate cancer
  • laser capture micro-dissection
  • microarray analysis
  • signal transduction

ASJC Scopus subject areas

  • Urology
  • Oncology
  • Medicine(all)

Cite this

Ross, A. E., Marchionni, L., Vuica-Ross, M., Cheadle, C., Fan, J., Berman, D. M., & Schaeffer, E. M. (2011). Gene expression pathways of high grade localized prostate cancer. Prostate, 71(14), 1568-1578. https://doi.org/10.1002/pros.21373