TY - JOUR
T1 - Gene expression of mycobacterium tuberculosis putative transcription factors whib1-7 in redox environments
AU - Larsson, Christer
AU - Luna, Brian
AU - Ammerman, Nicole C.
AU - Maiga, Mamoudou
AU - Agarwal, Nisheeth
AU - Bishai, William R.
N1 - Funding Information:
The Swedish Research Council (VR) and Swedish Society for Medical Research (SSMF) are gratefully acknowledged for their support (awarded to CL). This research was also supported by NIH AI36973 and AI37856 (awarded to WRB) and the Howard Hughes Medical Institute (WRB).
PY - 2012/7/19
Y1 - 2012/7/19
N2 - The seven WhiB proteins of Mycobacterium tuberculosis (M.tb) are widely believed to be redox-sensing transcription factors due to their binding of iron-sulfur clusters and similarities to DNA binding proteins. Here, we explored the nature of this hypothesized relationship. We exposed M.tb to physiologic conditions such as gradual hypoxia, nitric oxide (NO), cyclic AMP and in vivo conditions, and measured transcription of the whiB genes. We found whiB3 to be induced both by hypoxia and NO, whiB7 to be induced in macrophage-like cells, and whiB4 to be induced in mouse lung. Cyclic AMP induced whiB1,-2, -4, -6 and -7. Our data indicate that the M.tb whiB genes are induced independently by various stimuli which may add versatility to their suggested redox-sensing properties.
AB - The seven WhiB proteins of Mycobacterium tuberculosis (M.tb) are widely believed to be redox-sensing transcription factors due to their binding of iron-sulfur clusters and similarities to DNA binding proteins. Here, we explored the nature of this hypothesized relationship. We exposed M.tb to physiologic conditions such as gradual hypoxia, nitric oxide (NO), cyclic AMP and in vivo conditions, and measured transcription of the whiB genes. We found whiB3 to be induced both by hypoxia and NO, whiB7 to be induced in macrophage-like cells, and whiB4 to be induced in mouse lung. Cyclic AMP induced whiB1,-2, -4, -6 and -7. Our data indicate that the M.tb whiB genes are induced independently by various stimuli which may add versatility to their suggested redox-sensing properties.
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U2 - 10.1371/journal.pone.0037516
DO - 10.1371/journal.pone.0037516
M3 - Article
C2 - 22829866
AN - SCOPUS:84864050604
SN - 1932-6203
VL - 7
JO - PloS one
JF - PloS one
IS - 7
M1 - e37516
ER -