Gene expression in donor corneal endothelium

John D Gottsch, Gerami D. Seitzman, Elliott H. Margulies, Amanda L. Bowers, Allison J. Michels, Saurabh Saha, Albert Jun, Walter J. Stark, Sammy H. Liu

Research output: Contribution to journalArticle

Abstract

Objective: To report gene expression profiles of normal human corneal endothelium with microarray analysis and serial analysis of gene expression (SAGE). Methods: Corneal endothelium was removed from normal human corneas obtained from eye banks. Total RNA was isolated and SAGE analysis was performed. The same RNA source was used to construct a complementary DNA library that was hybridized to microarrays containing 12558 transcripts. Results: A total of 9530 SAGE tags were sequenced, representing 4724 unique tags. Microarray analysis identified 542 distinct transcripts. A database of human corneal endothelial gene expression was compiled. Of the SAGE tags, 1720 matched known genes, 478 corresponded to expressed sequence tags, and 2526 had no known match to public databases. The 5 most abundantly expressed SAGE tags were cytochrome c oxidase subunit II, adenosine triphosphate synthase F0 subunit 6, carbonic anhydrase XII, 12S ribosomal RNA, and ferritin, heavy polypeptide 1. Thirty-four percent of the transcripts (n = 1616) were specific to the corneal endothelium, when compared with other publicly available SAGE libraries. The 5 most abundant unique tags were keratin 12, angiopoietinlike factor, annexin A8, and 2 tags with no match to the database. Many endothelial pump function enzymes were confirmed, including several plasma membrane Na+/K+ adenosine triphosphatases and a recently reported bicarbonate transporter. Conclusions: Corneal endothelial gene expression profiles by the current analysis provide an understanding of endothelial metabolism, structure, and function; enable comparisons to diseased endothelium; and provide baseline data that may lead to the discovery of novel endothelial genes.

Original languageEnglish (US)
Pages (from-to)252-258
Number of pages7
JournalArchives of Ophthalmology
Volume121
Issue number2
StatePublished - Feb 1 2003

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Corneal Endothelium
Gene Expression
Databases
Microarray Analysis
Gene Library
Transcriptome
Keratin-12
Eye Banks
RNA
Annexins
Expressed Sequence Tags
Ferritins
Bicarbonates
Cornea
Genes
Endothelium
Adenosine Triphosphatases
Complementary DNA
Adenosine Triphosphate
Cell Membrane

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Gottsch, J. D., Seitzman, G. D., Margulies, E. H., Bowers, A. L., Michels, A. J., Saha, S., ... Liu, S. H. (2003). Gene expression in donor corneal endothelium. Archives of Ophthalmology, 121(2), 252-258.

Gene expression in donor corneal endothelium. / Gottsch, John D; Seitzman, Gerami D.; Margulies, Elliott H.; Bowers, Amanda L.; Michels, Allison J.; Saha, Saurabh; Jun, Albert; Stark, Walter J.; Liu, Sammy H.

In: Archives of Ophthalmology, Vol. 121, No. 2, 01.02.2003, p. 252-258.

Research output: Contribution to journalArticle

Gottsch, JD, Seitzman, GD, Margulies, EH, Bowers, AL, Michels, AJ, Saha, S, Jun, A, Stark, WJ & Liu, SH 2003, 'Gene expression in donor corneal endothelium', Archives of Ophthalmology, vol. 121, no. 2, pp. 252-258.
Gottsch JD, Seitzman GD, Margulies EH, Bowers AL, Michels AJ, Saha S et al. Gene expression in donor corneal endothelium. Archives of Ophthalmology. 2003 Feb 1;121(2):252-258.
Gottsch, John D ; Seitzman, Gerami D. ; Margulies, Elliott H. ; Bowers, Amanda L. ; Michels, Allison J. ; Saha, Saurabh ; Jun, Albert ; Stark, Walter J. ; Liu, Sammy H. / Gene expression in donor corneal endothelium. In: Archives of Ophthalmology. 2003 ; Vol. 121, No. 2. pp. 252-258.
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