Gene delivery by pullulan derivatives in brain capillary endothelial cells for protein secretion

Louiza Bohn Thomsen, Jacek Lichota, Kwang Sik Kim, Torben Moos

Research output: Contribution to journalArticle

Abstract

The blood-brain barrier (BBB) formed by brain capillary endothelial cells protects the brain against potentially harmful substances present in the circulation, but also restricts exogenous substances such as pharmacologically acting drugs or proteins from entering the brain. A novel and rather unchallenged approach to allow proteins to enter the brain is gene therapy based on delivery of genetic material into brain capillary endothelial cells. In theory in vivo transfection will allow protein expression and secretion from brain capillary endothelial cells and further into the brain. This would denote a new paradigm for therapy to transport proteins across the BBB. The aim of this study was to investigate the possibility to use brain capillary endothelial cells as factories for recombinant protein production. Non-viral gene carriers were prepared from pullulan, a polysaccharide, and spermine, a naturally occurring polyamine that were additionally conjugated with plasmid DNA. We were able to transfect rat brain endothelial cells (RBE4s) and human brain microvascular endothelial cells (HBMECs). Transfection of HBMECs with pullulan-spermine conjugated with plasmid DNA bearing cDNA encoding human growth hormone 1 (hGH1), led to secretion of hGH1 protein into the growth medium. Hence, the pullulan-spermine delivery system is a very promising method for delivering DNA to brain endothelial cells with potential for using these cells as factories for secretion of proteins.

Original languageEnglish (US)
Pages (from-to)45-50
Number of pages6
JournalJournal of Controlled Release
Volume151
Issue number1
DOIs
StatePublished - Apr 10 2011

Fingerprint

Endothelial Cells
Brain
Genes
Proteins
Spermine
Human Growth Hormone
Blood-Brain Barrier
Transfection
pullulan
DNA
Plasmids
Polyamines
Recombinant Proteins
Genetic Therapy
Polysaccharides
Carrier Proteins
Complementary DNA
Growth
Pharmaceutical Preparations

Keywords

  • blood-brain barrier
  • Gene expression
  • Gene therapy
  • Pullulan
  • Spermine

ASJC Scopus subject areas

  • Pharmaceutical Science

Cite this

Gene delivery by pullulan derivatives in brain capillary endothelial cells for protein secretion. / Thomsen, Louiza Bohn; Lichota, Jacek; Kim, Kwang Sik; Moos, Torben.

In: Journal of Controlled Release, Vol. 151, No. 1, 10.04.2011, p. 45-50.

Research output: Contribution to journalArticle

Thomsen, Louiza Bohn ; Lichota, Jacek ; Kim, Kwang Sik ; Moos, Torben. / Gene delivery by pullulan derivatives in brain capillary endothelial cells for protein secretion. In: Journal of Controlled Release. 2011 ; Vol. 151, No. 1. pp. 45-50.
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