Gel filtration in 6 M guanidine hydrochloride of the α-subunit (and its fragments) of the receptor for immunoglobulin E

Nirbhay Kumar, Henry Metzger

Research output: Contribution to journalArticle

Abstract

The mol. wts of the α-chain of the receptor for immunoglobulin E and several of its enzyme-cleaved fragments have been evaluated by gel filtration on Sepharose 6B in 6 M guanidine HCl. The mol. wt of α-chains treated with endoglycosidase was 30% less than that of untreated α-chains. α-Chains digested with papain eluted in a single peak with a mol. wt approximately one-half of that of undigested α-chains. The results support the proposal that papain cleaves α-chains into two fragments of similar size [Goetze et al. (1981) Biochemistry 20, 6341-6349].

Original languageEnglish (US)
Pages (from-to)1561-1567
Number of pages7
JournalMolecular Immunology
Volume19
Issue number12
DOIs
StatePublished - 1982
Externally publishedYes

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IgE Receptors
Papain
Guanidine
Gel Chromatography
Glycoside Hydrolases
Biochemistry
Sepharose
Enzymes

ASJC Scopus subject areas

  • Molecular Biology
  • Immunology

Cite this

Gel filtration in 6 M guanidine hydrochloride of the α-subunit (and its fragments) of the receptor for immunoglobulin E. / Kumar, Nirbhay; Metzger, Henry.

In: Molecular Immunology, Vol. 19, No. 12, 1982, p. 1561-1567.

Research output: Contribution to journalArticle

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