Gel filtration chromatography (size exclusion chromatography) of proteins

Krisna C. Duong-Ly, Sandra B. Gabelli

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

The protocol described here allows the student to construct a standard curve for a gel filtration column with a separation range of 5-250 kD. The size (hydrodynamic radius) of a protein species stable in a buffer containing Tris-HCl, NaCl, and DTT is determined using this column. Modifications may be made to the buffer to accommodate the protein of interest and the separation range of the column.

Original languageEnglish (US)
Title of host publicationLaboratory Methods in Enzymology
Subtitle of host publicationProtein Part C
PublisherAcademic Press Inc.
Pages105-114
Number of pages10
ISBN (Print)9780124201194
DOIs
StatePublished - 2014

Publication series

NameMethods in Enzymology
Volume541
ISSN (Print)0076-6879
ISSN (Electronic)1557-7988

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Keywords

  • Column volume
  • Gel filtration chromatography
  • Gel filtration column
  • Size exclusion chromatography
  • Void volume

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Cite this

Duong-Ly, K. C., & Gabelli, S. B. (2014). Gel filtration chromatography (size exclusion chromatography) of proteins. In Laboratory Methods in Enzymology: Protein Part C (pp. 105-114). (Methods in Enzymology; Vol. 541). Academic Press Inc.. https://doi.org/10.1016/B978-0-12-420119-4.00009-4