@inbook{885887e8dd5042f29936e5f094cb8572,
title = "Gel filtration chromatography (size exclusion chromatography) of proteins",
abstract = "The protocol described here allows the student to construct a standard curve for a gel filtration column with a separation range of 5-250 kD. The size (hydrodynamic radius) of a protein species stable in a buffer containing Tris-HCl, NaCl, and DTT is determined using this column. Modifications may be made to the buffer to accommodate the protein of interest and the separation range of the column.",
keywords = "Column volume, Gel filtration chromatography, Gel filtration column, Size exclusion chromatography, Void volume",
author = "Duong-Ly, {Krisna C.} and Gabelli, {Sandra B.}",
year = "2014",
doi = "10.1016/B978-0-12-420119-4.00009-4",
language = "English (US)",
isbn = "9780124201194",
series = "Methods in Enzymology",
publisher = "Academic Press Inc.",
pages = "105--114",
booktitle = "Laboratory Methods in Enzymology",
}