GATA3 up-regulation associated with surface expression of CD294/CRTH2: A unique feature of human Th cells

Umberto De Fanis, Francesca Mori, Rebecca J. Kurnat, Kyung Lee Won, Maria Bova, N Franklin Adkinson, Vincenzo Casolaro

Research output: Contribution to journalArticle

Abstract

GATA-3 and T-box expressed in T cells (T-bet) play central roles in Th-cell development and function. Consistently, studies in mice document their selective expression in Th1 and Th2 cells, respectively. In contrast, it is not clear whether these genes are regulated in human Th cells. Here we show that T-bet expression is polarized to a comparable degree in human and mouse Th-cell cultures, while only mouse GATA3 is subject to substantial regulation. This did not reflect differential skewing efficiency in human versus mouse cultures, as these contained similar frequencies of IFN-γ- and IL-4-producing cells. However, GATA-3 was expressed at significantly higher levels in human IL-4-producing cells enriched via capture with monoclonal antibodies (mAbs) against the PGD2 receptor, CRTH2, the best selective Th2-cell surface marker to date. Along with increased IL-4 and GATA-3, CRTH2+ Th cells isolated from Th2-skewed cultures or the circulating memory pool exhibited markedly decreased IFN-γ and T-bet expression. Thus, the human GATA-3 gene is not regulated in response to polarizing signals that are sufficient to direct Th2-specific expression in mouse cells. This postulates the involvement of an additional level of complexity in the regulation of human GATA-3 expression and stresses the existence of nontrivial differences in the regulation of human versus mouse T-cell function.

Original languageEnglish (US)
Pages (from-to)4343-4350
Number of pages8
JournalBlood
Volume109
Issue number10
DOIs
StatePublished - May 15 2007

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T-cells
Up-Regulation
Interleukin-4
Th2 Cells
Genes
T-Lymphocytes
Cell culture
Monoclonal Antibodies
Th1 Cells
Data storage equipment
Cell Culture Techniques
Efficiency

ASJC Scopus subject areas

  • Hematology

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GATA3 up-regulation associated with surface expression of CD294/CRTH2 : A unique feature of human Th cells. / De Fanis, Umberto; Mori, Francesca; Kurnat, Rebecca J.; Won, Kyung Lee; Bova, Maria; Adkinson, N Franklin; Casolaro, Vincenzo.

In: Blood, Vol. 109, No. 10, 15.05.2007, p. 4343-4350.

Research output: Contribution to journalArticle

De Fanis, Umberto ; Mori, Francesca ; Kurnat, Rebecca J. ; Won, Kyung Lee ; Bova, Maria ; Adkinson, N Franklin ; Casolaro, Vincenzo. / GATA3 up-regulation associated with surface expression of CD294/CRTH2 : A unique feature of human Th cells. In: Blood. 2007 ; Vol. 109, No. 10. pp. 4343-4350.
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