Gastrointestinal microphysiological systems

Sarah E. Blutt, James R. Broughman, Winnie Zou, Xi Lei Zeng, Umesh C. Karandikar, Julie In, Nicholas Zachos, Olga N Kovbasnjuk, Mark Donowitz, Mary K. Estes

Research output: Contribution to journalReview article

Abstract

Gastrointestinal diseases are a significant health care and economic burden. Prevention and treatment of these diseases have been limited by the available human biologic models. Microphysiological systems comprise organ-specific human cultures that recapitulate many structural, biological, and functional properties of the organ in smaller scale including aspects of flow, shear stress and chemical gradients. The development of intestinal microphysiological system platforms represents a critical component in improving our understanding, prevention, and treatment of gastrointestinal diseases. This minireview discusses: shortcomings of classical cell culture models of the gastrointestinal tract; human intestinal enteroids as a new model and their advantages compared to cell lines; why intestinal microphysiological systems are needed; potential functional uses of intestinal microphysiological systems in areas of drug development and modeling acute and chronic diseases; and current challenges in the development of intestinal microphysiological systems. Impact statement: The development of a gastrointestinal MPS has the potential to facilitate the understanding of GI physiology. An ultimate goal is the integration of the intestinal MPS with other organ MPS. The development and characterization of nontransformed human intestinal cultures for use in MPS have progressed significantly since the inception of the MPS program in 2012, and these cultures are a key component of advancing MPS. Continued efforts are needed to optimize MPS to comprehensively and accurately recapitulate the complexity of the intestinal epithelium within intestinal tissue. These systems will need to include peristalsis, flow, and oxygen gradients, with incorporation of vascular, immune, and nerve cells. Regional cellular organization of crypt and villus areas will also be necessary to better model complete intestinal structure.

Original languageEnglish (US)
Pages (from-to)1633-1642
Number of pages10
JournalExperimental Biology and Medicine
Volume242
Issue number16
DOIs
StatePublished - Oct 1 2017

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Gastrointestinal Diseases
Peristalsis
Biological Models
Drug Design
Physiology
Acute Disease
Intestinal Mucosa
Health care
Cell culture
Neurons
Blood Vessels
Gastrointestinal Tract
Shear stress
Chronic Disease
Cell Culture Techniques
Cells
Economics
Tissue
Oxygen
Delivery of Health Care

Keywords

  • Human intestinal enteroids
  • microphysiological systems

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Blutt, S. E., Broughman, J. R., Zou, W., Zeng, X. L., Karandikar, U. C., In, J., ... Estes, M. K. (2017). Gastrointestinal microphysiological systems. Experimental Biology and Medicine, 242(16), 1633-1642. https://doi.org/10.1177/1535370217710638

Gastrointestinal microphysiological systems. / Blutt, Sarah E.; Broughman, James R.; Zou, Winnie; Zeng, Xi Lei; Karandikar, Umesh C.; In, Julie; Zachos, Nicholas; Kovbasnjuk, Olga N; Donowitz, Mark; Estes, Mary K.

In: Experimental Biology and Medicine, Vol. 242, No. 16, 01.10.2017, p. 1633-1642.

Research output: Contribution to journalReview article

Blutt, SE, Broughman, JR, Zou, W, Zeng, XL, Karandikar, UC, In, J, Zachos, N, Kovbasnjuk, ON, Donowitz, M & Estes, MK 2017, 'Gastrointestinal microphysiological systems', Experimental Biology and Medicine, vol. 242, no. 16, pp. 1633-1642. https://doi.org/10.1177/1535370217710638
Blutt SE, Broughman JR, Zou W, Zeng XL, Karandikar UC, In J et al. Gastrointestinal microphysiological systems. Experimental Biology and Medicine. 2017 Oct 1;242(16):1633-1642. https://doi.org/10.1177/1535370217710638
Blutt, Sarah E. ; Broughman, James R. ; Zou, Winnie ; Zeng, Xi Lei ; Karandikar, Umesh C. ; In, Julie ; Zachos, Nicholas ; Kovbasnjuk, Olga N ; Donowitz, Mark ; Estes, Mary K. / Gastrointestinal microphysiological systems. In: Experimental Biology and Medicine. 2017 ; Vol. 242, No. 16. pp. 1633-1642.
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