Gas-Phase Scrambling of Disulfide Bonds during Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Analysis

Liang Zhao, Ruben T. Almaraz, Fan Xiang, Jerry L. Hedrick, Andreas H. Franz

Research output: Contribution to journalArticle

Abstract

Evidence for photo-induced radical disulfide bond scrambling in the gas phase during matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) is described. The phenomenon was observed during the analysis of tryptic peptides from insulin and was confirmed in the determination of disulfide bonds in the rhamnose-binding lectin SEL24K from the Chinook salmon Oncorhynchus tshawytscha. A possible mechanism for this surprising scrambling is proposed. Despite this finding, the disulfide bond pattern in SEL24K was assigned unambiguously by a multi-enzyme digestion strategy in combination with MALDI mass spectrometry. The pattern was found to be symmetrical in the tandem repeat sequence of SEL24K. To the best of our knowledge, this is the first report of disulfide bond scrambling in the gas phase during MALDI-MS analysis. This observation has important ramifications for unambiguous assignment of disulfide bonds.

Original languageEnglish (US)
Pages (from-to)1603-1616
Number of pages14
JournalJournal of the American Society for Mass Spectrometry
Volume20
Issue number9
DOIs
StatePublished - Sep 2009
Externally publishedYes

Fingerprint

Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
Disulfides
Ionization
Mass spectrometry
Desorption
Gases
Lasers
Salmon
Rhamnose
Tandem Repeat Sequences
Lectins
Digestion
Mass Spectrometry
Insulin
Peptides
Enzymes

ASJC Scopus subject areas

  • Structural Biology
  • Spectroscopy

Cite this

Gas-Phase Scrambling of Disulfide Bonds during Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Analysis. / Zhao, Liang; Almaraz, Ruben T.; Xiang, Fan; Hedrick, Jerry L.; Franz, Andreas H.

In: Journal of the American Society for Mass Spectrometry, Vol. 20, No. 9, 09.2009, p. 1603-1616.

Research output: Contribution to journalArticle

Zhao, Liang ; Almaraz, Ruben T. ; Xiang, Fan ; Hedrick, Jerry L. ; Franz, Andreas H. / Gas-Phase Scrambling of Disulfide Bonds during Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Analysis. In: Journal of the American Society for Mass Spectrometry. 2009 ; Vol. 20, No. 9. pp. 1603-1616.
@article{87ca3437105e430784f2462d34d020e4,
title = "Gas-Phase Scrambling of Disulfide Bonds during Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Analysis",
abstract = "Evidence for photo-induced radical disulfide bond scrambling in the gas phase during matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) is described. The phenomenon was observed during the analysis of tryptic peptides from insulin and was confirmed in the determination of disulfide bonds in the rhamnose-binding lectin SEL24K from the Chinook salmon Oncorhynchus tshawytscha. A possible mechanism for this surprising scrambling is proposed. Despite this finding, the disulfide bond pattern in SEL24K was assigned unambiguously by a multi-enzyme digestion strategy in combination with MALDI mass spectrometry. The pattern was found to be symmetrical in the tandem repeat sequence of SEL24K. To the best of our knowledge, this is the first report of disulfide bond scrambling in the gas phase during MALDI-MS analysis. This observation has important ramifications for unambiguous assignment of disulfide bonds.",
author = "Liang Zhao and Almaraz, {Ruben T.} and Fan Xiang and Hedrick, {Jerry L.} and Franz, {Andreas H.}",
year = "2009",
month = "9",
doi = "10.1016/j.jasms.2009.04.021",
language = "English (US)",
volume = "20",
pages = "1603--1616",
journal = "Journal of the American Society for Mass Spectrometry",
issn = "1044-0305",
publisher = "Springer New York",
number = "9",

}

TY - JOUR

T1 - Gas-Phase Scrambling of Disulfide Bonds during Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Analysis

AU - Zhao, Liang

AU - Almaraz, Ruben T.

AU - Xiang, Fan

AU - Hedrick, Jerry L.

AU - Franz, Andreas H.

PY - 2009/9

Y1 - 2009/9

N2 - Evidence for photo-induced radical disulfide bond scrambling in the gas phase during matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) is described. The phenomenon was observed during the analysis of tryptic peptides from insulin and was confirmed in the determination of disulfide bonds in the rhamnose-binding lectin SEL24K from the Chinook salmon Oncorhynchus tshawytscha. A possible mechanism for this surprising scrambling is proposed. Despite this finding, the disulfide bond pattern in SEL24K was assigned unambiguously by a multi-enzyme digestion strategy in combination with MALDI mass spectrometry. The pattern was found to be symmetrical in the tandem repeat sequence of SEL24K. To the best of our knowledge, this is the first report of disulfide bond scrambling in the gas phase during MALDI-MS analysis. This observation has important ramifications for unambiguous assignment of disulfide bonds.

AB - Evidence for photo-induced radical disulfide bond scrambling in the gas phase during matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) is described. The phenomenon was observed during the analysis of tryptic peptides from insulin and was confirmed in the determination of disulfide bonds in the rhamnose-binding lectin SEL24K from the Chinook salmon Oncorhynchus tshawytscha. A possible mechanism for this surprising scrambling is proposed. Despite this finding, the disulfide bond pattern in SEL24K was assigned unambiguously by a multi-enzyme digestion strategy in combination with MALDI mass spectrometry. The pattern was found to be symmetrical in the tandem repeat sequence of SEL24K. To the best of our knowledge, this is the first report of disulfide bond scrambling in the gas phase during MALDI-MS analysis. This observation has important ramifications for unambiguous assignment of disulfide bonds.

UR - http://www.scopus.com/inward/record.url?scp=69549120036&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=69549120036&partnerID=8YFLogxK

U2 - 10.1016/j.jasms.2009.04.021

DO - 10.1016/j.jasms.2009.04.021

M3 - Article

C2 - 19535267

AN - SCOPUS:69549120036

VL - 20

SP - 1603

EP - 1616

JO - Journal of the American Society for Mass Spectrometry

JF - Journal of the American Society for Mass Spectrometry

SN - 1044-0305

IS - 9

ER -