Gas chromatography/tandem mass spectrometry detection of extracellular kynurenine and related metabolites in normal and lesioned rat brain

Francesca M. Notarangelo, Hui Qiu Wu, Anthony Macherone, David Graham, Robert Schwarcz

Research output: Contribution to journalArticle

Abstract

We describe here a gas chromatography-tandem mass spectrometry (GC/MS/MS) method for the sensitive and concurrent determination of extracellular tryptophan and the kynurenine pathway metabolites kynurenine, 3-hydroxykynurenine (3-HK), and quinolinic acid (QUIN) in rat brain. This metabolic cascade is increasingly linked to the pathophysiology of several neurological and psychiatric diseases. Methodological refinements, including optimization of MS conditions and the addition of deuterated standards, resulted in assay linearity to the low nanomolar range. Measured in samples obtained by striatal microdialysis in vivo, basal levels of tryptophan, kynurenine, and QUIN were 415, 89, and 8 nM, respectively, but 3-HK levels were below the limit of detection (

Original languageEnglish (US)
Pages (from-to)573-581
Number of pages9
JournalAnalytical Biochemistry
Volume421
Issue number2
DOIs
StatePublished - Feb 15 2012

Fingerprint

Kynurenine
Metabolites
Tandem Mass Spectrometry
Gas chromatography
Gas Chromatography-Mass Spectrometry
Quinolinic Acid
Mass spectrometry
Rats
Brain
Tryptophan
Corpus Striatum
Microdialysis
Psychiatry
Limit of Detection
Assays
3-hydroxykynurenine

Keywords

  • 3-Hydroxykynurenine
  • Microdialysis
  • Quinolinic acid
  • Selective reaction monitoring
  • Tandem mass spectrometry
  • Tryptophan

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology
  • Cell Biology

Cite this

Gas chromatography/tandem mass spectrometry detection of extracellular kynurenine and related metabolites in normal and lesioned rat brain. / Notarangelo, Francesca M.; Wu, Hui Qiu; Macherone, Anthony; Graham, David; Schwarcz, Robert.

In: Analytical Biochemistry, Vol. 421, No. 2, 15.02.2012, p. 573-581.

Research output: Contribution to journalArticle

Notarangelo, Francesca M. ; Wu, Hui Qiu ; Macherone, Anthony ; Graham, David ; Schwarcz, Robert. / Gas chromatography/tandem mass spectrometry detection of extracellular kynurenine and related metabolites in normal and lesioned rat brain. In: Analytical Biochemistry. 2012 ; Vol. 421, No. 2. pp. 573-581.
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