Gas chromatography-mass spectrometry analysis of 2-amino-1-methyl-6-phenyl-imidazo[4,5-b]pyridine in urine and feces

M. D. Friesen; L. Garren; J. C. Bereziat; F. Kadlubar; D. Lin

doi:10.1289/ehp.9399179

Gas chromatography-mass spectrometry analysis of 2-amino-1-methyl-6-phenyl-imidazo[4,5-b]pyridine in urine and feces

M. D. Friesen, L. Garren, J. C. Bereziat, F. Kadlubar, D. Lin

Research output: Contribution to journal › Article › peer-review

16 Scopus citations

Abstract

A method has been developed to measure levels of 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) excreted in urine and feces. The method involves organic solvent extraction, derivatization to form electron-capturing bis-pentafluorobenzyl derivatives, and analysis by gas chromatography-negative ion chemical ionization mass spectrometry using a deuterium- labeled internal standard. The method can detect PhIP at levels of less than 1 ng/g in rat urine (5 ng/24 hr) and 5 ng/g (wet weight) in rat feces (50 ng/24 hr). Sprague-Dawley rats given a single 50 μg dose of PhIP by gavage excreted an average of 0.6% of the dose in the urine and 25% of the dose in the feces as unchanged PhIP, in the first 4 days after treatment. To make this method applicable for the analyses of biological fluids of PhIP-exposed human subjects, it is now being improved by using immunoaffinity chromatography.

Original language	English (US)
Pages (from-to)	179-181
Number of pages	3
Journal	Environmental health perspectives
Volume	99
DOIs	https://doi.org/10.1289/ehp.9399179
State	Published - 1993
Externally published	Yes

ASJC Scopus subject areas

Public Health, Environmental and Occupational Health
Health, Toxicology and Mutagenesis

Access to Document

10.1289/ehp.9399179

Cite this

@article{72a3d9b9ba424df5ad4a1e2cb9c18adc,

title = "Gas chromatography-mass spectrometry analysis of 2-amino-1-methyl-6-phenyl-imidazo[4,5-b]pyridine in urine and feces",

abstract = "A method has been developed to measure levels of 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) excreted in urine and feces. The method involves organic solvent extraction, derivatization to form electron-capturing bis-pentafluorobenzyl derivatives, and analysis by gas chromatography-negative ion chemical ionization mass spectrometry using a deuterium- labeled internal standard. The method can detect PhIP at levels of less than 1 ng/g in rat urine (5 ng/24 hr) and 5 ng/g (wet weight) in rat feces (50 ng/24 hr). Sprague-Dawley rats given a single 50 μg dose of PhIP by gavage excreted an average of 0.6% of the dose in the urine and 25% of the dose in the feces as unchanged PhIP, in the first 4 days after treatment. To make this method applicable for the analyses of biological fluids of PhIP-exposed human subjects, it is now being improved by using immunoaffinity chromatography.",

author = "Friesen, {M. D.} and L. Garren and Bereziat, {J. C.} and F. Kadlubar and D. Lin",

year = "1993",

doi = "10.1289/ehp.9399179",

language = "English (US)",

volume = "99",

pages = "179--181",

journal = "Environmental health perspectives",

issn = "0091-6765",

publisher = "Public Health Services, US Dept of Health and Human Services",

}

TY - JOUR

T1 - Gas chromatography-mass spectrometry analysis of 2-amino-1-methyl-6-phenyl-imidazo[4,5-b]pyridine in urine and feces

AU - Friesen, M. D.

AU - Garren, L.

AU - Bereziat, J. C.

AU - Kadlubar, F.

AU - Lin, D.

PY - 1993

Y1 - 1993

N2 - A method has been developed to measure levels of 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) excreted in urine and feces. The method involves organic solvent extraction, derivatization to form electron-capturing bis-pentafluorobenzyl derivatives, and analysis by gas chromatography-negative ion chemical ionization mass spectrometry using a deuterium- labeled internal standard. The method can detect PhIP at levels of less than 1 ng/g in rat urine (5 ng/24 hr) and 5 ng/g (wet weight) in rat feces (50 ng/24 hr). Sprague-Dawley rats given a single 50 μg dose of PhIP by gavage excreted an average of 0.6% of the dose in the urine and 25% of the dose in the feces as unchanged PhIP, in the first 4 days after treatment. To make this method applicable for the analyses of biological fluids of PhIP-exposed human subjects, it is now being improved by using immunoaffinity chromatography.

AB - A method has been developed to measure levels of 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) excreted in urine and feces. The method involves organic solvent extraction, derivatization to form electron-capturing bis-pentafluorobenzyl derivatives, and analysis by gas chromatography-negative ion chemical ionization mass spectrometry using a deuterium- labeled internal standard. The method can detect PhIP at levels of less than 1 ng/g in rat urine (5 ng/24 hr) and 5 ng/g (wet weight) in rat feces (50 ng/24 hr). Sprague-Dawley rats given a single 50 μg dose of PhIP by gavage excreted an average of 0.6% of the dose in the urine and 25% of the dose in the feces as unchanged PhIP, in the first 4 days after treatment. To make this method applicable for the analyses of biological fluids of PhIP-exposed human subjects, it is now being improved by using immunoaffinity chromatography.

UR - http://www.scopus.com/inward/record.url?scp=0027294839&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027294839&partnerID=8YFLogxK

U2 - 10.1289/ehp.9399179

DO - 10.1289/ehp.9399179

M3 - Article

C2 - 8319618

AN - SCOPUS:0027294839

SN - 0091-6765

VL - 99

SP - 179

EP - 181

JO - Environmental health perspectives

JF - Environmental health perspectives

ER -

Gas chromatography-mass spectrometry analysis of 2-amino-1-methyl-6-phenyl-imidazo[4,5-b]pyridine in urine and feces

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this