Gas chromatographic determination of pentoxifylline and its major metabolites in human breast milk

Michael T. Bauza, Robert V. Smith, D. Elaine Knutson, Frank R. Witter

Research output: Contribution to journalArticle

Abstract

A method has been developed for determination of the xanthine drug, pentoxifylline, and three of its metabolites (a secondary alcohol and two carboxylic acids) in human milk. The method requires pre-extraction with hexane to remove lipids followed by extraction with dichloromethane or dichloromethane-isopropanol (4:1). Absolute extraction recoveries were between 76-90%. Pentoxifylline and its alcohol metabolite (as the trifluoroacetate) and the carboxylic acid metabolites (as ethyl esters) were measured in separate gas chromatographic steps using a nitrogen detector. Determinations of pentoxifylline and its three metabolites were 96-99% accurate and standard deviations of 5-10% were observed for samples at or above the lower practical sensitivity limit (10 ng/ml) for the assay. Pentoxifylline and its metabolites were stable in breast milk for three weeks when stored at -15°C.

Original languageEnglish (US)
Pages (from-to)61-69
Number of pages9
JournalJournal of Chromatography B: Biomedical Sciences and Applications
Volume310
Issue numberC
DOIs
StatePublished - 1984

ASJC Scopus subject areas

  • Chemistry(all)

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