Gangliosides' analysis by MALDI-ion mobility MS

Shelley N. Jackson; Benoit Colsch; Thomas Egan; Ernest K. Lewis; J. Albert Schultz; Amina S. Woods

doi:10.1039/c0an00732c

Gangliosides' analysis by MALDI-ion mobility MS

Shelley N. Jackson, Benoit Colsch, Thomas Egan, Ernest K. Lewis, J. Albert Schultz, Amina S. Woods

Research output: Contribution to journal › Article › peer-review

38 Scopus citations

Abstract

The combination of ion mobility with matrix-assisted laser desorption/ionization allows for the rapid separation and analysis of biomolecules in complex mixtures (such as tissue sections and cellular extracts), as isobaric lipid, peptide, and oligonucleotide molecular ions are pre-separated in the mobility cell before mass analysis. In this study, MALDI-IM MS is used to analyze gangliosides, a class of complex glycosphingolipids that has different degrees of sialylation. Both GD1a and GD1b, structural isomers, were studied to see the effects on gas-phase structure depending upon the localization of the sialic acids. A total ganglioside extract from mouse brain was also analyzed to measure the effectiveness of ion mobility to separate out the different ganglioside species in a complex mixture.

Original language	English (US)
Pages (from-to)	463-466
Number of pages	4
Journal	The Analyst
Volume	136
Issue number	3
DOIs	https://doi.org/10.1039/c0an00732c
State	Published - Feb 7 2011
Externally published	Yes

ASJC Scopus subject areas

Analytical Chemistry
Spectroscopy
Electrochemistry
Biochemistry
Environmental Chemistry

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abstract = "The combination of ion mobility with matrix-assisted laser desorption/ionization allows for the rapid separation and analysis of biomolecules in complex mixtures (such as tissue sections and cellular extracts), as isobaric lipid, peptide, and oligonucleotide molecular ions are pre-separated in the mobility cell before mass analysis. In this study, MALDI-IM MS is used to analyze gangliosides, a class of complex glycosphingolipids that has different degrees of sialylation. Both GD1a and GD1b, structural isomers, were studied to see the effects on gas-phase structure depending upon the localization of the sialic acids. A total ganglioside extract from mouse brain was also analyzed to measure the effectiveness of ion mobility to separate out the different ganglioside species in a complex mixture.",

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AU - Jackson, Shelley N.

AU - Colsch, Benoit

AU - Egan, Thomas

AU - Lewis, Ernest K.

AU - Schultz, J. Albert

AU - Woods, Amina S.

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AB - The combination of ion mobility with matrix-assisted laser desorption/ionization allows for the rapid separation and analysis of biomolecules in complex mixtures (such as tissue sections and cellular extracts), as isobaric lipid, peptide, and oligonucleotide molecular ions are pre-separated in the mobility cell before mass analysis. In this study, MALDI-IM MS is used to analyze gangliosides, a class of complex glycosphingolipids that has different degrees of sialylation. Both GD1a and GD1b, structural isomers, were studied to see the effects on gas-phase structure depending upon the localization of the sialic acids. A total ganglioside extract from mouse brain was also analyzed to measure the effectiveness of ion mobility to separate out the different ganglioside species in a complex mixture.

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Gangliosides' analysis by MALDI-ion mobility MS

Abstract

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