Gangliosides' analysis by MALDI-ion mobility MS

Shelley N. Jackson, Benoit Colsch, Thomas Egan, Ernest K. Lewis, J. Albert Schultz, Amina S. Woods

Research output: Contribution to journalArticlepeer-review

Abstract

The combination of ion mobility with matrix-assisted laser desorption/ionization allows for the rapid separation and analysis of biomolecules in complex mixtures (such as tissue sections and cellular extracts), as isobaric lipid, peptide, and oligonucleotide molecular ions are pre-separated in the mobility cell before mass analysis. In this study, MALDI-IM MS is used to analyze gangliosides, a class of complex glycosphingolipids that has different degrees of sialylation. Both GD1a and GD1b, structural isomers, were studied to see the effects on gas-phase structure depending upon the localization of the sialic acids. A total ganglioside extract from mouse brain was also analyzed to measure the effectiveness of ion mobility to separate out the different ganglioside species in a complex mixture.

Original languageEnglish (US)
Pages (from-to)463-466
Number of pages4
JournalThe Analyst
Volume136
Issue number3
DOIs
StatePublished - Feb 7 2011
Externally publishedYes

ASJC Scopus subject areas

  • Analytical Chemistry
  • Spectroscopy
  • Electrochemistry
  • Biochemistry
  • Environmental Chemistry

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