Gangliosides' analysis by MALDI-ion mobility MS

Shelley N. Jackson, Benoit Colsch, Thomas Egan, Ernest K. Lewis, J. Albert Schultz, Amina S. Woods

Research output: Contribution to journalArticle

Abstract

The combination of ion mobility with matrix-assisted laser desorption/ionization allows for the rapid separation and analysis of biomolecules in complex mixtures (such as tissue sections and cellular extracts), as isobaric lipid, peptide, and oligonucleotide molecular ions are pre-separated in the mobility cell before mass analysis. In this study, MALDI-IM MS is used to analyze gangliosides, a class of complex glycosphingolipids that has different degrees of sialylation. Both GD1a and GD1b, structural isomers, were studied to see the effects on gas-phase structure depending upon the localization of the sialic acids. A total ganglioside extract from mouse brain was also analyzed to measure the effectiveness of ion mobility to separate out the different ganglioside species in a complex mixture.

Original languageEnglish (US)
Pages (from-to)463-466
Number of pages4
JournalThe Analyst
Volume136
Issue number3
DOIs
StatePublished - Feb 7 2011
Externally publishedYes

Fingerprint

Gangliosides
Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
Ions
Complex Mixtures
ion
Sialic Acids
Glycosphingolipids
Oligonucleotides
Biomolecules
Phase structure
Isomers
peptide
Lipids
Peptides
Ionization
brain
Desorption
Brain
desorption
Lasers

ASJC Scopus subject areas

  • Analytical Chemistry
  • Spectroscopy
  • Electrochemistry
  • Biochemistry
  • Environmental Chemistry

Cite this

Jackson, S. N., Colsch, B., Egan, T., Lewis, E. K., Schultz, J. A., & Woods, A. S. (2011). Gangliosides' analysis by MALDI-ion mobility MS. The Analyst, 136(3), 463-466. https://doi.org/10.1039/c0an00732c

Gangliosides' analysis by MALDI-ion mobility MS. / Jackson, Shelley N.; Colsch, Benoit; Egan, Thomas; Lewis, Ernest K.; Schultz, J. Albert; Woods, Amina S.

In: The Analyst, Vol. 136, No. 3, 07.02.2011, p. 463-466.

Research output: Contribution to journalArticle

Jackson, SN, Colsch, B, Egan, T, Lewis, EK, Schultz, JA & Woods, AS 2011, 'Gangliosides' analysis by MALDI-ion mobility MS', The Analyst, vol. 136, no. 3, pp. 463-466. https://doi.org/10.1039/c0an00732c
Jackson SN, Colsch B, Egan T, Lewis EK, Schultz JA, Woods AS. Gangliosides' analysis by MALDI-ion mobility MS. The Analyst. 2011 Feb 7;136(3):463-466. https://doi.org/10.1039/c0an00732c
Jackson, Shelley N. ; Colsch, Benoit ; Egan, Thomas ; Lewis, Ernest K. ; Schultz, J. Albert ; Woods, Amina S. / Gangliosides' analysis by MALDI-ion mobility MS. In: The Analyst. 2011 ; Vol. 136, No. 3. pp. 463-466.
@article{6a4c0adabde54feb9ea2c891bfd5ab3f,
title = "Gangliosides' analysis by MALDI-ion mobility MS",
abstract = "The combination of ion mobility with matrix-assisted laser desorption/ionization allows for the rapid separation and analysis of biomolecules in complex mixtures (such as tissue sections and cellular extracts), as isobaric lipid, peptide, and oligonucleotide molecular ions are pre-separated in the mobility cell before mass analysis. In this study, MALDI-IM MS is used to analyze gangliosides, a class of complex glycosphingolipids that has different degrees of sialylation. Both GD1a and GD1b, structural isomers, were studied to see the effects on gas-phase structure depending upon the localization of the sialic acids. A total ganglioside extract from mouse brain was also analyzed to measure the effectiveness of ion mobility to separate out the different ganglioside species in a complex mixture.",
author = "Jackson, {Shelley N.} and Benoit Colsch and Thomas Egan and Lewis, {Ernest K.} and Schultz, {J. Albert} and Woods, {Amina S.}",
year = "2011",
month = "2",
day = "7",
doi = "10.1039/c0an00732c",
language = "English (US)",
volume = "136",
pages = "463--466",
journal = "The Analyst",
issn = "0003-2654",
publisher = "Royal Society of Chemistry",
number = "3",

}

TY - JOUR

T1 - Gangliosides' analysis by MALDI-ion mobility MS

AU - Jackson, Shelley N.

AU - Colsch, Benoit

AU - Egan, Thomas

AU - Lewis, Ernest K.

AU - Schultz, J. Albert

AU - Woods, Amina S.

PY - 2011/2/7

Y1 - 2011/2/7

N2 - The combination of ion mobility with matrix-assisted laser desorption/ionization allows for the rapid separation and analysis of biomolecules in complex mixtures (such as tissue sections and cellular extracts), as isobaric lipid, peptide, and oligonucleotide molecular ions are pre-separated in the mobility cell before mass analysis. In this study, MALDI-IM MS is used to analyze gangliosides, a class of complex glycosphingolipids that has different degrees of sialylation. Both GD1a and GD1b, structural isomers, were studied to see the effects on gas-phase structure depending upon the localization of the sialic acids. A total ganglioside extract from mouse brain was also analyzed to measure the effectiveness of ion mobility to separate out the different ganglioside species in a complex mixture.

AB - The combination of ion mobility with matrix-assisted laser desorption/ionization allows for the rapid separation and analysis of biomolecules in complex mixtures (such as tissue sections and cellular extracts), as isobaric lipid, peptide, and oligonucleotide molecular ions are pre-separated in the mobility cell before mass analysis. In this study, MALDI-IM MS is used to analyze gangliosides, a class of complex glycosphingolipids that has different degrees of sialylation. Both GD1a and GD1b, structural isomers, were studied to see the effects on gas-phase structure depending upon the localization of the sialic acids. A total ganglioside extract from mouse brain was also analyzed to measure the effectiveness of ion mobility to separate out the different ganglioside species in a complex mixture.

UR - http://www.scopus.com/inward/record.url?scp=78751525363&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=78751525363&partnerID=8YFLogxK

U2 - 10.1039/c0an00732c

DO - 10.1039/c0an00732c

M3 - Article

C2 - 21113547

AN - SCOPUS:78751525363

VL - 136

SP - 463

EP - 466

JO - The Analyst

JF - The Analyst

SN - 0003-2654

IS - 3

ER -