Gamma endonuclease of Micrococcus luteus: Action on irradiated DNA

T. J. Jorgensen, E. A. Furlong, W. D. Henner

Research output: Contribution to journalArticle

Abstract

Gamma endonuclease is a Mg2+-independent enzyme of Micrococcus luteus that recognizes and cleaves DNA at a variety of altered pyrimidines produced by ionizing radiation. The production of enzyme-recognizable sites (ERS) by ionizing radiation under different irradiation conditions was measured. Ionizing radiation produced the greatest number of ERS when irradiations were performed under anoxic conditions in the presence of the free radical scavenger KI. Since dihydrothymine is a major pyrimidine lesion produced in DNA during anoxic irradiation, the ability of γ endonuclease to excise this lesion was assessed. Dihydrothymine was released from DNA irradiated under anoxic conditions in a radiation dose-dependent manner, consistent with γ endonuclease's known DNA glycosylase activity. Gamma endonuclease was also shown to cleave heavily uv-irradiated DNA. When the sequence specificity of γ-endonuclease cleavage was studied using uv-irradiated DNA, cleavage was seen specifically at cytosines. The identity of this enzyme-recognizable cytosine photoproduct is not known.

Original languageEnglish (US)
Pages (from-to)556-566
Number of pages11
JournalRadiation Research
Volume114
Issue number3
StatePublished - 1988
Externally publishedYes

Fingerprint

Micrococcus luteus
Endonucleases
deoxyribonucleic acid
Ionizing Radiation
enzymes
Cytosine
DNA
Enzymes
ionizing radiation
irradiation
cytosine
pyrimidines
DNA Glycosylases
anaerobic conditions
Pyrimidines
lesions
DNA Cleavage
Free Radical Scavengers
cleavage
glycosylases

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biophysics
  • Radiology Nuclear Medicine and imaging
  • Radiation

Cite this

Jorgensen, T. J., Furlong, E. A., & Henner, W. D. (1988). Gamma endonuclease of Micrococcus luteus: Action on irradiated DNA. Radiation Research, 114(3), 556-566.

Gamma endonuclease of Micrococcus luteus : Action on irradiated DNA. / Jorgensen, T. J.; Furlong, E. A.; Henner, W. D.

In: Radiation Research, Vol. 114, No. 3, 1988, p. 556-566.

Research output: Contribution to journalArticle

Jorgensen, TJ, Furlong, EA & Henner, WD 1988, 'Gamma endonuclease of Micrococcus luteus: Action on irradiated DNA', Radiation Research, vol. 114, no. 3, pp. 556-566.
Jorgensen TJ, Furlong EA, Henner WD. Gamma endonuclease of Micrococcus luteus: Action on irradiated DNA. Radiation Research. 1988;114(3):556-566.
Jorgensen, T. J. ; Furlong, E. A. ; Henner, W. D. / Gamma endonuclease of Micrococcus luteus : Action on irradiated DNA. In: Radiation Research. 1988 ; Vol. 114, No. 3. pp. 556-566.
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