Galectin-3 induces endothelial cell morphogenesis and angiogenesis

Pratima Nangia-Makker, Yuichiro Honjo, Rebecca Sarvis, Shiro Akahani, Victor Hogan, Kenneth J. Pienta, Avraham Raz

Research output: Contribution to journalArticlepeer-review


Increasing evidence suggests that carbohydrate-binding proteins play an essential role in tumor growth and metastasis. However, conflicting results on their function in the regulation of cell proliferation and differentiation during angiogenesis have been reported. We have examined the role of galectin-3 in the regulation of human umbilical vein endothelial cell proliferation, differentiation, migration, and neovascularization. Galectin-3, a carbohydrate-binding protein, with specificity for type 1 and 11 ABH blood group epitopes and polylactosamine glycan containing cell surface glycoproteins, is the major nonintegrin cellular laminin-binding protein. Because galectin-3 expression was shown to be associated in some tumor systems with metastasis, we questioned whether it induces endothelial cell morphogenesis. Here we show that galectin-3 affects chemotaxis and morphology and stimulates capillary tube formation of HUV-EC-C in vitro and angiogenesis in vivo. Endothelial cell morphogenesis is a carbohydrate-dependent process, as it is neutralized by specific sugars and antibodies. These findings demonstrate that endothelial cell surface carbohydrate recognition event(s) can induce a signaling cascade leading to the differentiation and angiogenesis of endothelial cells.

Original languageEnglish (US)
Pages (from-to)899-909
Number of pages11
JournalAmerican Journal of Pathology
Issue number3
StatePublished - 2000

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Fingerprint Dive into the research topics of 'Galectin-3 induces endothelial cell morphogenesis and angiogenesis'. Together they form a unique fingerprint.

Cite this