Galect1ns from ectothermic vertebrates and invertebrates: molecular structure and carbohydrate-binding specificities

H. Ahmed, D. E. Henrikson, N. O'Learv, Mario Antonio Bianchet, Mario L Amzel, J. Hirabayashi, K. Kasai, G. R. Vasla

Research output: Contribution to journalArticle

Abstract

Galectins are a family of soluble β-gaiactoside-binding lectins present in vertebrates, invertebrates, and fungi, which exhibit a typical motif of conserved amino acids in their carbohydrate recognition domain(s) (CRDs). In order to gain insight on galectin structure-function relationships we characterized the molecular organization and carbohydrate specificity of galectins from ectothermic vertebrates (amphibians and fish) and invertebrates (tunicates, echinoderms and sponges). On SDS-PAGE under reducing conditions, galectins from most species appeared as multiple bands ranging from 14.5 kDa to 67 kDa. Galectins from amphibians and fish species exhibited galectin-1-like "conserved" type CRDs (Ahmed & Vasta, 1994 Glycobiology 4: 545-549), as determined from their primary structures and carbohydrate-binding specificities. The C. elegans 16 kDa galectin exhibits a "variable" type CRD with a novel carbohydrate specificity. The structural basis of the carbohydrate specificities characterized was elucidated through the determination of the structures or homology modeling.

Original languageEnglish (US)
JournalFASEB Journal
Volume12
Issue number8
StatePublished - 1998

Fingerprint

Galectins
carbohydrate binding
Invertebrates
Molecular Structure
chemical structure
Molecular structure
Vertebrates
invertebrates
vertebrates
Carbohydrates
carbohydrates
amphibians
Amphibians
Fish
Tunicata
Fishes
structure-activity relationships
Echinodermata
Porifera
fish

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

Galect1ns from ectothermic vertebrates and invertebrates : molecular structure and carbohydrate-binding specificities. / Ahmed, H.; Henrikson, D. E.; O'Learv, N.; Bianchet, Mario Antonio; Amzel, Mario L; Hirabayashi, J.; Kasai, K.; Vasla, G. R.

In: FASEB Journal, Vol. 12, No. 8, 1998.

Research output: Contribution to journalArticle

Ahmed, H. ; Henrikson, D. E. ; O'Learv, N. ; Bianchet, Mario Antonio ; Amzel, Mario L ; Hirabayashi, J. ; Kasai, K. ; Vasla, G. R. / Galect1ns from ectothermic vertebrates and invertebrates : molecular structure and carbohydrate-binding specificities. In: FASEB Journal. 1998 ; Vol. 12, No. 8.
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