Galactomannan Antigenemia and Antigenuria in Aspergillosis: Studies in Patients and Experimentally Infected Rabbits

Bertrand Dupont, Margret Huber, Sang J. Kim, John E. Bennett

Research output: Contribution to journalArticle

Abstract

Purified galactomannan (GM) from Aspergillus fumigatus was used in both a radioimmunoassay and an enzyme-linked immunoassay for antigen detection: Results of the two tests seemed interchangeable. By one or both assays, GM was detected in serum from four of 12 rabbits lethally infected with A. fumigatus in concentrations ranging from 108 to 356 ng/ml. Serum antigen was detected in only two of 12 patients with invasive aspergillosis. Results of assay for GM in urine were far more encouraging. Urinary GM was detectable throughout the course of lethal aspergillosis in all 16 rabbits, in concentrations of 24–1,900 ng/ml. Urine from seven of 13 patients with invasive aspergillosis had GM concentrations of 1–83 ng/ml. Antigen excretion roughly paralleled extent of disease.

Original languageEnglish (US)
Pages (from-to)1-11
Number of pages11
JournalJournal of Infectious Diseases
Volume155
Issue number1
DOIs
StatePublished - 1987
Externally publishedYes

Fingerprint

Aspergillosis
Rabbits
Aspergillus fumigatus
Antigens
Urine
Serum
Immunoenzyme Techniques
Radioimmunoassay
galactomannan

ASJC Scopus subject areas

  • Infectious Diseases
  • Immunology and Allergy
  • Immunology
  • Public Health, Environmental and Occupational Health

Cite this

Galactomannan Antigenemia and Antigenuria in Aspergillosis : Studies in Patients and Experimentally Infected Rabbits. / Dupont, Bertrand; Huber, Margret; Kim, Sang J.; Bennett, John E.

In: Journal of Infectious Diseases, Vol. 155, No. 1, 1987, p. 1-11.

Research output: Contribution to journalArticle

Dupont, Bertrand ; Huber, Margret ; Kim, Sang J. ; Bennett, John E. / Galactomannan Antigenemia and Antigenuria in Aspergillosis : Studies in Patients and Experimentally Infected Rabbits. In: Journal of Infectious Diseases. 1987 ; Vol. 155, No. 1. pp. 1-11.
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