TY - JOUR
T1 - GABAergic Projection Neurons Route Selective Olfactory Inputs to Specific Higher-Order Neurons
AU - Liang, Liang
AU - Li, Yulong
AU - Potter, Christopher J.
AU - Yizhar, Ofer
AU - Deisseroth, Karl
AU - Tsien, Richard W.
AU - Luo, Liqun
N1 - Funding Information:
We thank Y. Chou, B. Dickson, V. Jayaraman, T. Lee, L. Looger, K. Wehner, X. Yu, the Bloomington Stock Center, the Vienna Drosophila RNAi Center, the Developmental Studies Hybridoma Bank, and the BACPAC Resources Center for fly stocks, reagents, and antibodies; S. Sinha, D. Profitt, and D. Luginbuhl for technical assistance; K. Beier, X. Chen, T. Clandinin, X. Gao, N. Makki, A. Mizrahi, T. Mosca, and R. Wilson for providing critiques of the manuscript; M. Schnitzer for support; and G. Miesenböck for communicating data prior to publication. This work is supported by an NIH grant (R01-DC005982 to L. Luo). L. Liang has been supported by a Stanford Graduate Fellowship and a Lubert Stryer Stanford Interdisciplinary Graduate Fellowship. L. Luo is an investigator of the Howard Hughes Medical Institute.
PY - 2013/9/4
Y1 - 2013/9/4
N2 - We characterize an inhibitory circuit motif in the Drosophila olfactory system, parallel inhibition, which differs from feedforward or feedback inhibition. Excitatory and GABAergic inhibitory projection neurons (ePNs and iPNs) each receive input from antennal lobe glomeruli and send parallel output to the lateral horn, a higher center implicated in regulating innate olfactory behavior. Ca2+ imaging of specific lateral horn neurons as an olfactory readout revealed that iPNs selectively suppressed food-related odor responses, but spared signal transmission from pheromone channels. Coapplying food odorant did not affect pheromone signal transmission, suggesting that the differential effects likely result from connection specificity of iPNs, rather than a generalized inhibitory tone. Ca2+ responses in the ePN axon terminals show no detectable suppression by iPNs, arguing against presynaptic inhibition as a primary mechanism. The parallel inhibition motif may provide specificity in inhibition to funnel specific olfactory information, such as food and pheromone, into distinct downstream circuits
AB - We characterize an inhibitory circuit motif in the Drosophila olfactory system, parallel inhibition, which differs from feedforward or feedback inhibition. Excitatory and GABAergic inhibitory projection neurons (ePNs and iPNs) each receive input from antennal lobe glomeruli and send parallel output to the lateral horn, a higher center implicated in regulating innate olfactory behavior. Ca2+ imaging of specific lateral horn neurons as an olfactory readout revealed that iPNs selectively suppressed food-related odor responses, but spared signal transmission from pheromone channels. Coapplying food odorant did not affect pheromone signal transmission, suggesting that the differential effects likely result from connection specificity of iPNs, rather than a generalized inhibitory tone. Ca2+ responses in the ePN axon terminals show no detectable suppression by iPNs, arguing against presynaptic inhibition as a primary mechanism. The parallel inhibition motif may provide specificity in inhibition to funnel specific olfactory information, such as food and pheromone, into distinct downstream circuits
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U2 - 10.1016/j.neuron.2013.06.014
DO - 10.1016/j.neuron.2013.06.014
M3 - Article
C2 - 24012005
AN - SCOPUS:84884183042
SN - 0896-6273
VL - 79
SP - 917
EP - 931
JO - Neuron
JF - Neuron
IS - 5
ER -