GABA- and glutamate-activated channels in green fluorescent protein- tagged gonadotropin-releasing hormone neurons in transgenic mice

Daniel J. Spergel, Ulrich Krüth, Daniel F Hanley, Rolf Sprengel, Peter H. Seeburg

Research output: Contribution to journalArticle

Abstract

Mice were generated expressing green fluorescent protein (GFP) under the control of the gonadotropin-releasing hormone (GnRH) promoter. Green fluorescence was observed in, and restricted to, GnRH-immunopositive neuronal somata in the olfactory bulb, ganglion terminale, septal nuclei, diagonal band of Broca (DBB), preoptic area (POA), and caudal hypothalamus, as well as GnRH neuronal dendrites and axons, including axon terminals in the median eminence and organum vasculosum of the lamina terminalis (OVLT). Whole-cell recordings from GFP-expressing GnRH neurons in the OVLT-POA-DBB region revealed a firing pattern among GFP-expressing GnRH neurons distinct from that of nonfluorescent neurons. Nucleated patches of GFP-expressing GnRH neurons exhibited pronounced responses to fast application of GABA and smaller responses to L-glutamate and AMPA. One-fifth of the nucleated patches responded to NMDA. The GABA-A, AMPA, and NMDA receptor channels on GnRH neurons mediating these responses may play a role in the modulation of GnRH secretory oscillations.

Original languageEnglish (US)
Pages (from-to)2037-2050
Number of pages14
JournalJournal of Neuroscience
Volume19
Issue number6
StatePublished - Mar 15 1999
Externally publishedYes

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Green Fluorescent Proteins
Gonadotropin-Releasing Hormone
gamma-Aminobutyric Acid
Transgenic Mice
Glutamic Acid
Neurons
Diagonal Band of Broca
Septal Nuclei
Preoptic Area
Median Eminence
alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid
AMPA Receptors
Olfactory Bulb
Presynaptic Terminals
Carisoprodol
Patch-Clamp Techniques
N-Methylaspartate
GABA-A Receptors
Dendrites
N-Methyl-D-Aspartate Receptors

Keywords

  • Diagonal band of Broca
  • GABA
  • GFP
  • Glutamate
  • GnRH
  • Hypothalamus
  • Median eminence
  • Organum vasculosum of the lamina terminalis
  • Preoptic area
  • Transgenic mice

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

GABA- and glutamate-activated channels in green fluorescent protein- tagged gonadotropin-releasing hormone neurons in transgenic mice. / Spergel, Daniel J.; Krüth, Ulrich; Hanley, Daniel F; Sprengel, Rolf; Seeburg, Peter H.

In: Journal of Neuroscience, Vol. 19, No. 6, 15.03.1999, p. 2037-2050.

Research output: Contribution to journalArticle

Spergel, Daniel J. ; Krüth, Ulrich ; Hanley, Daniel F ; Sprengel, Rolf ; Seeburg, Peter H. / GABA- and glutamate-activated channels in green fluorescent protein- tagged gonadotropin-releasing hormone neurons in transgenic mice. In: Journal of Neuroscience. 1999 ; Vol. 19, No. 6. pp. 2037-2050.
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