G protein subunits in lung cells

Charles W. Emala, Jianing Yang, Carol A. Hirshman, Michael A. Levine

    Research output: Contribution to journalArticle

    Abstract

    Many hormones and neurotransmitters bind to membrane-bound receptors that are coupled to signal generating enzymes or ion channels via signal transducing GTP-binding proteins termed G proteins. Although receptors and second messengers have been extensively studied in cells of the respiratory system, the G proteins responsible for the coupling of these proteins have not been well-characterized. Therefore, we used immunoblot analysis to determine expression of G protein α and β subunits in membranes prepared from cells and tissues of the respiratory system, including cultured canine tracheal epithelium, cleanly dissected canine tracheal smooth muscle, canine large conducting airways, and canine and human lung parenchyma. The two isoforms of Gsα (45 and 52 kDa) werepresent in all tissues, with a predominant expression of the 45 kDa isoform. Plasma membranes prepared from canine tracheal epithelium and muscle, and human lung parenchyma, contained greater amounts of Gsα than membranes prepared from canine bronchus and lung. Relative levels of immunoreactive Giα(2), Giα(3), Gq/G11α, β1 and β2 were similar in all of the tissues studied. By contrast, Goα was absent in cultured tracheal epithelium, and tracheal smooth muscle expressed greater amounts of Giα(2) compared to Giα(3). Specificity of G protein expression can provide one regulatory mechanism for functional biochemical pathways within cells. The demonstration of specific G protein subunits is the first step in the molecular characterization of the regulation of these pathways, both in normal tissues and in disease states.

    Original languageEnglish (US)
    Pages (from-to)593-602
    Number of pages10
    JournalLife Sciences
    Volume55
    Issue number8
    DOIs
    StatePublished - 1994

    Keywords

    • epithelium
    • G protein
    • lung
    • trachealis

    ASJC Scopus subject areas

    • Pharmacology

    Fingerprint Dive into the research topics of 'G protein subunits in lung cells'. Together they form a unique fingerprint.

  • Cite this

    Emala, C. W., Yang, J., Hirshman, C. A., & Levine, M. A. (1994). G protein subunits in lung cells. Life Sciences, 55(8), 593-602. https://doi.org/10.1016/0024-3205(94)00485-4