G protein signaling events are activated at the leading edge of chemotactic cells

Carole A. Parent, Brenda J. Blacklock, Wendy M. Froehlich, Douglas B. Murphy, Peter N Devreotes

Research output: Contribution to journalArticle

Abstract

Directional sensing by eukaryotic cells does not require polarization of chemoattractant receptors. The translocation of the PH domain-containing protein CRAC in D. discoideum to binding sites on the inner face of the plasma membrane reflects activation of the G protein-linked signaling system. Increments in chemoattractant elicit a uniform response around the cell periphery. Yet when cells are exposed to a gradient, the activation occurs selectively at the stimulated edge, even in immobilized cells. We propose that such localized activation, transmitted by the recruitment of cytosolic proteins, may be a general mechanism for gradient sensing by G protein- linked chemotactic systems including those involving chemotactic cytokines in leukocytes.

Original languageEnglish (US)
Pages (from-to)81-91
Number of pages11
JournalCell
Volume95
Issue number1
DOIs
StatePublished - Oct 2 1998

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GTP-Binding Proteins
Chemical activation
Formyl Peptide Receptor
Immobilized Cells
Chemotactic Factors
Eukaryotic Cells
Chemokines
Proteins
Leukocytes
Binding Sites
Cell Membrane
Cell membranes
Cells
Polarization
Pleckstrin Homology Domains

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

Cite this

G protein signaling events are activated at the leading edge of chemotactic cells. / Parent, Carole A.; Blacklock, Brenda J.; Froehlich, Wendy M.; Murphy, Douglas B.; Devreotes, Peter N.

In: Cell, Vol. 95, No. 1, 02.10.1998, p. 81-91.

Research output: Contribution to journalArticle

Parent, Carole A. ; Blacklock, Brenda J. ; Froehlich, Wendy M. ; Murphy, Douglas B. ; Devreotes, Peter N. / G protein signaling events are activated at the leading edge of chemotactic cells. In: Cell. 1998 ; Vol. 95, No. 1. pp. 81-91.
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