G protein mRNA mapped in rat brain by in situ hybridization

B. L. Largent, D. T. Jones, Randall R Reed, R. C A Pearson, Solomon H Snyder

Research output: Contribution to journalArticle

Abstract

Guanine nucleotide-binding regulatory proteins (G proteins) mediate many receptor-coupled signal transduction events. We have localized in rat brain by in situ hybridization the mRNA for the G protein subunits - G(αs), G(αo), and G(β). Oligonucleotide probes were radiolabeled by a technique that resulted in a probe of defined specific activity and uniform length. mRNA species encoding G(αs) and G(β) occur in high densities heterogeneously throughout the brain, especially in large neuronal cell bodies - e.g., hippocampal pyramidal cells, granule cells of the dentate gyrus, hypothalamic nuclei, and neurons of brainstem nuclei and the reticular formation. G(αo) mRNA has a more limited distribution and abundance, being detectable in the claustrum, endopiriform nucleus, habenula, hippocampal pyramidal cells, granule cells of the dentate gyrus, and cerebellar Purkinje cells.

Original languageEnglish (US)
Pages (from-to)2864-2868
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume85
Issue number8
Publication statusPublished - 1988

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ASJC Scopus subject areas

  • General
  • Genetics

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