Abstract
Guanine nucleotide-binding regulatory proteins (G proteins) mediate many receptor-coupled signal transduction events. We have localized in rat brain by in situ hybridization the mRNA for the G protein subunits - G(αs), G(αo), and G(β). Oligonucleotide probes were radiolabeled by a technique that resulted in a probe of defined specific activity and uniform length. mRNA species encoding G(αs) and G(β) occur in high densities heterogeneously throughout the brain, especially in large neuronal cell bodies - e.g., hippocampal pyramidal cells, granule cells of the dentate gyrus, hypothalamic nuclei, and neurons of brainstem nuclei and the reticular formation. G(αo) mRNA has a more limited distribution and abundance, being detectable in the claustrum, endopiriform nucleus, habenula, hippocampal pyramidal cells, granule cells of the dentate gyrus, and cerebellar Purkinje cells.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 2864-2868 |
| Number of pages | 5 |
| Journal | Proceedings of the National Academy of Sciences of the United States of America |
| Volume | 85 |
| Issue number | 8 |
| State | Published - 1988 |
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ASJC Scopus subject areas
- General
- Genetics
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G protein mRNA mapped in rat brain by in situ hybridization. / Largent, B. L.; Jones, D. T.; Reed, Randall R; Pearson, R. C A; Snyder, Solomon H.
In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 85, No. 8, 1988, p. 2864-2868.Research output: Contribution to journal › Article
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TY - JOUR
T1 - G protein mRNA mapped in rat brain by in situ hybridization
AU - Largent, B. L.
AU - Jones, D. T.
AU - Reed, Randall R
AU - Pearson, R. C A
AU - Snyder, Solomon H
PY - 1988
Y1 - 1988
N2 - Guanine nucleotide-binding regulatory proteins (G proteins) mediate many receptor-coupled signal transduction events. We have localized in rat brain by in situ hybridization the mRNA for the G protein subunits - G(αs), G(αo), and G(β). Oligonucleotide probes were radiolabeled by a technique that resulted in a probe of defined specific activity and uniform length. mRNA species encoding G(αs) and G(β) occur in high densities heterogeneously throughout the brain, especially in large neuronal cell bodies - e.g., hippocampal pyramidal cells, granule cells of the dentate gyrus, hypothalamic nuclei, and neurons of brainstem nuclei and the reticular formation. G(αo) mRNA has a more limited distribution and abundance, being detectable in the claustrum, endopiriform nucleus, habenula, hippocampal pyramidal cells, granule cells of the dentate gyrus, and cerebellar Purkinje cells.
AB - Guanine nucleotide-binding regulatory proteins (G proteins) mediate many receptor-coupled signal transduction events. We have localized in rat brain by in situ hybridization the mRNA for the G protein subunits - G(αs), G(αo), and G(β). Oligonucleotide probes were radiolabeled by a technique that resulted in a probe of defined specific activity and uniform length. mRNA species encoding G(αs) and G(β) occur in high densities heterogeneously throughout the brain, especially in large neuronal cell bodies - e.g., hippocampal pyramidal cells, granule cells of the dentate gyrus, hypothalamic nuclei, and neurons of brainstem nuclei and the reticular formation. G(αo) mRNA has a more limited distribution and abundance, being detectable in the claustrum, endopiriform nucleus, habenula, hippocampal pyramidal cells, granule cells of the dentate gyrus, and cerebellar Purkinje cells.
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UR - http://www.scopus.com/inward/citedby.url?scp=0023943572&partnerID=8YFLogxK
M3 - Article
C2 - 3128797
AN - SCOPUS:0023943572
VL - 85
SP - 2864
EP - 2868
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
SN - 0027-8424
IS - 8
ER -