TY - JOUR
T1 - G protein mRNA mapped in rat brain by in situ hybridization
AU - Largent, B. L.
AU - Jones, D. T.
AU - Reed, R. R.
AU - Pearson, R. C.A.
AU - Snyder, S. H.
PY - 1988
Y1 - 1988
N2 - Guanine nucleotide-binding regulatory proteins (G proteins) mediate many receptor-coupled signal transduction events. We have localized in rat brain by in situ hybridization the mRNA for the G protein subunits - G(αs), G(αo), and G(β). Oligonucleotide probes were radiolabeled by a technique that resulted in a probe of defined specific activity and uniform length. mRNA species encoding G(αs) and G(β) occur in high densities heterogeneously throughout the brain, especially in large neuronal cell bodies - e.g., hippocampal pyramidal cells, granule cells of the dentate gyrus, hypothalamic nuclei, and neurons of brainstem nuclei and the reticular formation. G(αo) mRNA has a more limited distribution and abundance, being detectable in the claustrum, endopiriform nucleus, habenula, hippocampal pyramidal cells, granule cells of the dentate gyrus, and cerebellar Purkinje cells.
AB - Guanine nucleotide-binding regulatory proteins (G proteins) mediate many receptor-coupled signal transduction events. We have localized in rat brain by in situ hybridization the mRNA for the G protein subunits - G(αs), G(αo), and G(β). Oligonucleotide probes were radiolabeled by a technique that resulted in a probe of defined specific activity and uniform length. mRNA species encoding G(αs) and G(β) occur in high densities heterogeneously throughout the brain, especially in large neuronal cell bodies - e.g., hippocampal pyramidal cells, granule cells of the dentate gyrus, hypothalamic nuclei, and neurons of brainstem nuclei and the reticular formation. G(αo) mRNA has a more limited distribution and abundance, being detectable in the claustrum, endopiriform nucleus, habenula, hippocampal pyramidal cells, granule cells of the dentate gyrus, and cerebellar Purkinje cells.
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U2 - 10.1073/pnas.85.8.2864
DO - 10.1073/pnas.85.8.2864
M3 - Article
C2 - 3128797
AN - SCOPUS:0023943572
VL - 85
SP - 2864
EP - 2868
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
SN - 0027-8424
IS - 8
ER -