G-protein-linked signal transduction systems control development in Dictyostelium

R. L. Johnson, R. Gundersen, P. Lilly, G. S. Pitt, M. Pupillo, T. J. Sun, R. A. Vaughan, P. N. Devreotes

Research output: Contribution to journalArticle

Abstract

G-protein-linked cAMP receptors play an essential role in Dictyostelium development. The cAMP receptors are proposed to have seven transmembrane domains and a cytoplasmic C-terminal region. Overexpression of the receptor in cells, when the endogenous receptor is not present, results in a 10- to 50-fold increase in cAMP-binding sites. Antisense cell lines, which lack cAMP receptors, do not enter the developmental program. Ligand-induced phosphorylation is proposed to occur on serine and threonine residues in the receptor C-terminus. The kinetics of receptor phosphorylation and dephosphorylation correlate closely with the shift of receptor mobility and the adaptation of several cAMP-induced responses. Two α-subunits, G-α-1 and G-α-2, have been cloned and specific antisera developed against each. Both subunits are expressed as multiple RNAs with different developmental time courses. The mutant Frigid A has a functional defect in G-α-2 which prevents it from entering development. We propose that G-protein-linked receptor systems will be a major component in the development of many organisms.

Original languageEnglish (US)
Pages (from-to)75-80
Number of pages6
JournalDevelopment
Volume107
Issue numberSUPPL.
StatePublished - Dec 1 1989

Keywords

  • G-protein
  • cAMP receptors
  • dictyostelium
  • phosphorylation

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology

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    Johnson, R. L., Gundersen, R., Lilly, P., Pitt, G. S., Pupillo, M., Sun, T. J., Vaughan, R. A., & Devreotes, P. N. (1989). G-protein-linked signal transduction systems control development in Dictyostelium. Development, 107(SUPPL.), 75-80.