G protein β subunit-null mutants are impaired in phagocytosis and chemotaxis due to inappropriate regulation of the actin cytoskeleton

Barbara Peracino, Jane Borleis, Tian Jin, Monika Westphal, Jean Marc Schwartz, Lijun Wu, Enrico Bracco, Günther Gerisch, Peter Devreotes, Salvatore Bozzaro

Research output: Contribution to journalArticle

Abstract

Chemotaxis and phagocytosis are basically similar in cells of the immune system and in Dictyostelium amebae. Deletion of the unique G protein β subunit in D. discoideum impaired phagocytosis but had little effect on fluid-phase endocytosis, cytokinesis, or random motility. Constitutive expression of wild-type β subunit restored phagocytosis and normal development. Chemoattractants released by cells or bacteria trigger typical transient actin polymerization responses in wild-type cells. In β subunit- null cells, and in a series of β subunit point mutants, these responses were impaired to a degree that correlated with the defect in phagocytosis. Image analysis of green fluorescent protein-actin transfected cells showed that β subunit-null cells were defective in reshaping the actin network into a phagocytic cup, and eventually a phagosome, in response to particle attachment. Our results indicate that signaling through heterotrimeric G proteins is required for regulating the actin cytoskeleton during phagocytic uptake, as previously shown for chemotaxis. Inhibitors of phospholipase C and intracellular Ca2+ mobilization inhibited phagocytosis, suggesting the possible involvement of these effectors in the process.

Original languageEnglish (US)
Pages (from-to)1529-1537
Number of pages9
JournalJournal of Cell Biology
Volume141
Issue number7
DOIs
StatePublished - Jun 29 1998

ASJC Scopus subject areas

  • Cell Biology

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    Peracino, B., Borleis, J., Jin, T., Westphal, M., Schwartz, J. M., Wu, L., Bracco, E., Gerisch, G., Devreotes, P., & Bozzaro, S. (1998). G protein β subunit-null mutants are impaired in phagocytosis and chemotaxis due to inappropriate regulation of the actin cytoskeleton. Journal of Cell Biology, 141(7), 1529-1537. https://doi.org/10.1083/jcb.141.7.1529