Fyn binds to and phosphorylates T cell immunoglobulin and mucin domain-1 (Tim-1)

Miranda L. Curtiss, Bruce S. Hostager, Elizabeth Stepniak, Melody Singh, Natalie Manhica, Judit Knisz, Geri Traver, Paul D. Rennert, John D. Colgan, Paul B Rothman

Research output: Contribution to journalArticle

Abstract

The gene encoding T cell immunoglobulin and mucin domain-1 (Tim-1) is linked to atopy and asthma susceptibility in mice and humans. Tim-1 is a transmembrane protein expressed on activated lymphocytes and appears to have a role as a co-stimulatory receptor in T cells. The protein has not been shown to have enzymatic activity but contains a site within its cytoplasmic tail predicted to be a target for tyrosine kinases. Here, we show that Tim-1 can associate with the kinase Fyn, a member of the Src family of tyrosine kinases. This association does not require Fyn's kinase activity and is independent of the phosphorylation of a conserved tyrosine present within the cytoplasmic tail of Tim-1. Fyn is necessary for phosphorylation of this tyrosine in Tim-1 and the phosphorylation of Tim-1 varies with the levels of Fyn present in cells. These data suggest a role for Fyn in the signaling downstream of Tim-1.

Original languageEnglish (US)
Pages (from-to)1424-1431
Number of pages8
JournalMolecular Immunology
Volume48
Issue number12-13
DOIs
StatePublished - Jul 2011
Externally publishedYes

Fingerprint

Mucin-1
Phosphorylation
T-Lymphocytes
Tyrosine
Tail
Phosphotransferases
src-Family Kinases
T-Cell Antigen Receptor
Protein-Tyrosine Kinases
Proteins
Asthma
Lymphocytes
Genes
Immunoglobulin Domains

Keywords

  • B cell
  • Fyn
  • Havcr-1
  • Signaling
  • Tim-1

ASJC Scopus subject areas

  • Molecular Biology
  • Immunology

Cite this

Curtiss, M. L., Hostager, B. S., Stepniak, E., Singh, M., Manhica, N., Knisz, J., ... Rothman, P. B. (2011). Fyn binds to and phosphorylates T cell immunoglobulin and mucin domain-1 (Tim-1). Molecular Immunology, 48(12-13), 1424-1431. https://doi.org/10.1016/j.molimm.2011.03.023

Fyn binds to and phosphorylates T cell immunoglobulin and mucin domain-1 (Tim-1). / Curtiss, Miranda L.; Hostager, Bruce S.; Stepniak, Elizabeth; Singh, Melody; Manhica, Natalie; Knisz, Judit; Traver, Geri; Rennert, Paul D.; Colgan, John D.; Rothman, Paul B.

In: Molecular Immunology, Vol. 48, No. 12-13, 07.2011, p. 1424-1431.

Research output: Contribution to journalArticle

Curtiss, ML, Hostager, BS, Stepniak, E, Singh, M, Manhica, N, Knisz, J, Traver, G, Rennert, PD, Colgan, JD & Rothman, PB 2011, 'Fyn binds to and phosphorylates T cell immunoglobulin and mucin domain-1 (Tim-1)', Molecular Immunology, vol. 48, no. 12-13, pp. 1424-1431. https://doi.org/10.1016/j.molimm.2011.03.023
Curtiss ML, Hostager BS, Stepniak E, Singh M, Manhica N, Knisz J et al. Fyn binds to and phosphorylates T cell immunoglobulin and mucin domain-1 (Tim-1). Molecular Immunology. 2011 Jul;48(12-13):1424-1431. https://doi.org/10.1016/j.molimm.2011.03.023
Curtiss, Miranda L. ; Hostager, Bruce S. ; Stepniak, Elizabeth ; Singh, Melody ; Manhica, Natalie ; Knisz, Judit ; Traver, Geri ; Rennert, Paul D. ; Colgan, John D. ; Rothman, Paul B. / Fyn binds to and phosphorylates T cell immunoglobulin and mucin domain-1 (Tim-1). In: Molecular Immunology. 2011 ; Vol. 48, No. 12-13. pp. 1424-1431.
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