Abstract
The gene encoding T cell immunoglobulin and mucin domain-1 (Tim-1) is linked to atopy and asthma susceptibility in mice and humans. Tim-1 is a transmembrane protein expressed on activated lymphocytes and appears to have a role as a co-stimulatory receptor in T cells. The protein has not been shown to have enzymatic activity but contains a site within its cytoplasmic tail predicted to be a target for tyrosine kinases. Here, we show that Tim-1 can associate with the kinase Fyn, a member of the Src family of tyrosine kinases. This association does not require Fyn's kinase activity and is independent of the phosphorylation of a conserved tyrosine present within the cytoplasmic tail of Tim-1. Fyn is necessary for phosphorylation of this tyrosine in Tim-1 and the phosphorylation of Tim-1 varies with the levels of Fyn present in cells. These data suggest a role for Fyn in the signaling downstream of Tim-1.
Original language | English (US) |
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Pages (from-to) | 1424-1431 |
Number of pages | 8 |
Journal | Molecular Immunology |
Volume | 48 |
Issue number | 12-13 |
DOIs | |
State | Published - Jul 2011 |
Externally published | Yes |
Keywords
- B cell
- Fyn
- Havcr-1
- Signaling
- Tim-1
ASJC Scopus subject areas
- Immunology
- Molecular Biology