Fyn binding protein, Fyb, interacts with mammalian actin binding protein, mAbp1

Ming Yuan, Lena Mogemark, Maria Fällman

Research output: Contribution to journalArticlepeer-review

Abstract

The immune cell specific protein Fyn-T binding protein (Fyb) has been identified as a target of the Yersinia antiphagocytic effector Yersinia outer protein H (YopH), but its role in macrophages is unknown. By using Fyb domains as bait to screen a mouse lymphoma cDNA library, we identified a novel interaction partner, mammalian actin binding protein 1 (mAbp1). We show that mAbp1 binds the Fyb N-terminal via its C-terminally located src homology 3 domain. The interaction between Fyb and mAbp1 is detected in macrophage lysates and the proteins co-localize with F-actin in the leading edge. Hence, mAbp1 is likely to constitute a downstream effector of Fyb involved in F-actin dynamics.

Original languageEnglish (US)
Pages (from-to)2339-2347
Number of pages9
JournalFEBS Letters
Volume579
Issue number11
DOIs
StatePublished - Apr 25 2005
Externally publishedYes

Keywords

  • F-actin
  • Fyn-T binding protein
  • Mammalian actin binding protein 1
  • Vasodilator-stimulated phosphoprotein
  • Yersinia outer protein H
  • src homology 3 domain

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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