TY - JOUR
T1 - Fyn binding protein, Fyb, interacts with mammalian actin binding protein, mAbp1
AU - Yuan, Ming
AU - Mogemark, Lena
AU - Fällman, Maria
N1 - Funding Information:
This work was supported by the Swedish Research Council, the Swedish Cancer Foundation, the King Gustavs Vth 80 year Foundation, the Medical Faculty Research Foundation at Umeå University. LM and MY are recipients of stipends from the J.C. Kempes Memorial Foundation. We thank Dr. Klemens Rottner and Dr. Jurgen Wehland for providing Fyb cDNA and Fyb antibodies, Dr Anders Bystrom for providing yeast strain 1974 and pGBKT7 (Ura) vector and Dr. Michael Way for providing pCB6-GFP vector.
PY - 2005/4/25
Y1 - 2005/4/25
N2 - The immune cell specific protein Fyn-T binding protein (Fyb) has been identified as a target of the Yersinia antiphagocytic effector Yersinia outer protein H (YopH), but its role in macrophages is unknown. By using Fyb domains as bait to screen a mouse lymphoma cDNA library, we identified a novel interaction partner, mammalian actin binding protein 1 (mAbp1). We show that mAbp1 binds the Fyb N-terminal via its C-terminally located src homology 3 domain. The interaction between Fyb and mAbp1 is detected in macrophage lysates and the proteins co-localize with F-actin in the leading edge. Hence, mAbp1 is likely to constitute a downstream effector of Fyb involved in F-actin dynamics.
AB - The immune cell specific protein Fyn-T binding protein (Fyb) has been identified as a target of the Yersinia antiphagocytic effector Yersinia outer protein H (YopH), but its role in macrophages is unknown. By using Fyb domains as bait to screen a mouse lymphoma cDNA library, we identified a novel interaction partner, mammalian actin binding protein 1 (mAbp1). We show that mAbp1 binds the Fyb N-terminal via its C-terminally located src homology 3 domain. The interaction between Fyb and mAbp1 is detected in macrophage lysates and the proteins co-localize with F-actin in the leading edge. Hence, mAbp1 is likely to constitute a downstream effector of Fyb involved in F-actin dynamics.
KW - F-actin
KW - Fyn-T binding protein
KW - Mammalian actin binding protein 1
KW - Vasodilator-stimulated phosphoprotein
KW - Yersinia outer protein H
KW - src homology 3 domain
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U2 - 10.1016/j.febslet.2005.03.031
DO - 10.1016/j.febslet.2005.03.031
M3 - Article
C2 - 15848169
AN - SCOPUS:17644378365
SN - 0014-5793
VL - 579
SP - 2339
EP - 2347
JO - FEBS Letters
JF - FEBS Letters
IS - 11
ER -