Further cholinergic aspects of carotid body chemotransduction of hypoxia in cats

Robert S Fitzgerald, Machiko Shirahata, Tohru Ide

Research output: Contribution to journalArticle

Abstract

From the 1930s into the 1970s, the role of acetylcholine (ACh) in the carotid body's chemotransduction of hypoxia was debated. Since the late 1970s, the issue has been pursued only intermittently or not at all. The purpose of this study was to test again with a new preparation the hypothesis that ACh is an excitatory neurotransmitter in the cat carotid body's chemotransduction of hypoxia. We tested the effect of the specific nicotinic blocker mecamylamine and the muscarinic blocker of all five muscarinic receptors, atropine. We further tested the effects of M1 and M2 muscarinic- receptor blockers. The carotid body region was selectively perfused with hypoxic Krebs-Ringer bicarbonate (KRB) solutions that were blocker free or contained varying doses of the blockers. Both mecamylamine and atropine reduced the response to hypoxic KRB in a dose-related manner. The M2 muscarinic-receptor blockers gallamine and AFDX 116 increased the response to hypoxic KRB, whereas the M1 muscarinic-receptor blocker pirenzepine reduced the response to hypoxic KRB. These data are consistent with an excitatory role for ACh in the carotid body chemotransduction of hypoxia in the cat.

Original languageEnglish (US)
Pages (from-to)819-827
Number of pages9
JournalJournal of Applied Physiology
Volume82
Issue number3
StatePublished - Mar 1997

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Carotid Body
Bicarbonates
Cholinergic Agents
Cats
Muscarinic M2 Receptors
Muscarinic M1 Receptors
Acetylcholine
Mecamylamine
Atropine
Gallamine Triethiodide
Pirenzepine
Body Regions
Muscarinic Receptors
Neurotransmitter Agents
Hypoxia

Keywords

  • acetylcholine
  • AFDX 116
  • atropine
  • gallamine
  • mecamylamine
  • pirenzepine

ASJC Scopus subject areas

  • Physiology
  • Endocrinology
  • Orthopedics and Sports Medicine
  • Physical Therapy, Sports Therapy and Rehabilitation

Cite this

Further cholinergic aspects of carotid body chemotransduction of hypoxia in cats. / Fitzgerald, Robert S; Shirahata, Machiko; Ide, Tohru.

In: Journal of Applied Physiology, Vol. 82, No. 3, 03.1997, p. 819-827.

Research output: Contribution to journalArticle

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