Further characterization of a 58 kDa Plasmodium berghei phosphoprotein as a cochaperone

Mark F. Wiser, Gregory J. Jennings, Pichart Uparanukraw, Alex Van Belkum, Leen Jan Van Doorn, Nirbhay Kumar

Research output: Contribution to journalArticle

Abstract

Molecular chaperones are important for proper protein folding during protein biogenesis. This report describes a protein from Plasmodium berghei which is 30% identical and 40% similar to a recently described mammalian cochaperone, or heat shock protein 70 interacting protein. The P. berghei cochaperone accumulates throughout the trophozoite stage and decreases during the schizont stage. The stage specific expression is consistent with its presumed role in protein folding or protein-protein interactions. The largest difference between the Plasmodium and mammalian sequences is a more extensive domain of imperfect glycine-glycine-methionine-proline (GGMP) tandem repeats in the parasite's cochaperone sequence. Immunofluorescence studies show that the protein is an abundant cytosolic protein of the parasite. However, antibodies raised against the GGMP repeat domain, which is also found in other parasite chaperones, react with both the parasite and host erythrocyte membrane. The reactivity with the host membrane suggests that the parasite exports molecular chaperones into the infected erythrocyte.

Original languageEnglish (US)
Pages (from-to)25-33
Number of pages9
JournalMolecular and Biochemical Parasitology
Volume83
Issue number1
DOIs
StatePublished - Dec 2 1996

Fingerprint

Plasmodium berghei
Phosphoproteins
Parasites
Glycine
Proteins
Molecular Chaperones
Protein Folding
Proline
Methionine
Schizonts
Trophozoites
Tandem Repeat Sequences
HSP70 Heat-Shock Proteins
Plasmodium
Erythrocyte Membrane
Fluorescent Antibody Technique
Erythrocytes
Membranes
Antibodies

Keywords

  • chaperone
  • cochaperone
  • heat shock protein
  • Plasmodium berghei
  • steroid hormone receptor
  • tandem repeats

ASJC Scopus subject areas

  • Molecular Biology
  • Parasitology

Cite this

Wiser, M. F., Jennings, G. J., Uparanukraw, P., Van Belkum, A., Van Doorn, L. J., & Kumar, N. (1996). Further characterization of a 58 kDa Plasmodium berghei phosphoprotein as a cochaperone. Molecular and Biochemical Parasitology, 83(1), 25-33. https://doi.org/10.1016/S0166-6851(96)02743-0

Further characterization of a 58 kDa Plasmodium berghei phosphoprotein as a cochaperone. / Wiser, Mark F.; Jennings, Gregory J.; Uparanukraw, Pichart; Van Belkum, Alex; Van Doorn, Leen Jan; Kumar, Nirbhay.

In: Molecular and Biochemical Parasitology, Vol. 83, No. 1, 02.12.1996, p. 25-33.

Research output: Contribution to journalArticle

Wiser, MF, Jennings, GJ, Uparanukraw, P, Van Belkum, A, Van Doorn, LJ & Kumar, N 1996, 'Further characterization of a 58 kDa Plasmodium berghei phosphoprotein as a cochaperone', Molecular and Biochemical Parasitology, vol. 83, no. 1, pp. 25-33. https://doi.org/10.1016/S0166-6851(96)02743-0
Wiser, Mark F. ; Jennings, Gregory J. ; Uparanukraw, Pichart ; Van Belkum, Alex ; Van Doorn, Leen Jan ; Kumar, Nirbhay. / Further characterization of a 58 kDa Plasmodium berghei phosphoprotein as a cochaperone. In: Molecular and Biochemical Parasitology. 1996 ; Vol. 83, No. 1. pp. 25-33.
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