TY - JOUR
T1 - Fungicides reduce the abundance of yeast-like symbionts and survival of white-backed planthopper sogatella furcifera (Homoptera: Delphacidae)
AU - Pang, Kun
AU - Dong, Shengzhang
AU - Hao, Peiying
AU - Chen, Tongtong
AU - Wang, Xinlong
AU - Yu, Xiaoping
AU - Lin, Huafeng
N1 - Funding Information:
Funding: This study was supported by the National Key Project of China (Grant No. 2017YFD02009), the National Natural Science Foundation of China (Grant Nos. 31901874, 31672026, 31171860, and 30900944), the Zhejiang Provincial Programs for Science and Technology Development (Grant No. 2019C02015), and the Zhejiang Provincial Natural Science Foundation of China (Grant No. LQ18C140002).
Funding Information:
This study was supported by the National Key Project of China (Grant No. 2017YFD02009), the National Natural Science Foundation of China (Grant Nos. 31901874, 31672026, 31171860, and 30900944), the Zhejiang Provincial Programs for Science and Technology Development (Grant No. 2019C02015), and the Zhejiang Provincial Natural Science Foundation of China (Grant No. LQ18C140002).
Publisher Copyright:
© 2020 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2020/4
Y1 - 2020/4
N2 - The white-backed planthopper (WBPH) Sogatella furcifera is one of the most harmful pests of rice in Southeast Asia. The fat body of WBPH harbors intracellular yeast-like symbionts (YLS). YLS are vertically transmitted to WBPH offspring by transovarial infection. YLS play an important role in the WBPH life cycle. YLS diversity and function have been extensively studied in the brown planthopper (BPH) and small brown planthopper but not in WBPH, even though a novel strategy for controlling the BPH based on suppressing YLS has been proposed. Here, using denaturing gradient gel electrophoresis, we identified 12 unique fungal sequences among YLS of WBPH, and five of them represented uncultured fungi. We then fed WBPH with rice plants treated with different fungicides [70% propineb wettable powder (WP) (PR), 70% propamocarb hydrochloride aqueous solution (AS) (PH), 25% trifloxystrobin and 50% tebuconazole water-dispersible granules (WG) (TT), 40% pyrimethanil suspension concentrate (SC) (PY), and 50% iprodione SC (IP)] and evaluated their effects on YLS abundance and WBPH survival rate. Both YLS abundance and adult WBPH survival rate were significantly decreased upon feeding fungicide-treated rice plants, and exposure to 50% IP resulted in the strongest reduction. The abundance of two Sf-YLS species (Ascomycetes symbiotes and Cla-like symbiotes) was significantly reduced upon exposure to 50% IP. The counts of Ascomycetes symbiotes, the most abundant YLS species, were also suppressed by the other fungicides tested. In conclusion, 50% IP was the most effective fungicide, reducing YLS abundance and WBPH survival rate under controlled conditions, suggesting its potential use to control WBPH.
AB - The white-backed planthopper (WBPH) Sogatella furcifera is one of the most harmful pests of rice in Southeast Asia. The fat body of WBPH harbors intracellular yeast-like symbionts (YLS). YLS are vertically transmitted to WBPH offspring by transovarial infection. YLS play an important role in the WBPH life cycle. YLS diversity and function have been extensively studied in the brown planthopper (BPH) and small brown planthopper but not in WBPH, even though a novel strategy for controlling the BPH based on suppressing YLS has been proposed. Here, using denaturing gradient gel electrophoresis, we identified 12 unique fungal sequences among YLS of WBPH, and five of them represented uncultured fungi. We then fed WBPH with rice plants treated with different fungicides [70% propineb wettable powder (WP) (PR), 70% propamocarb hydrochloride aqueous solution (AS) (PH), 25% trifloxystrobin and 50% tebuconazole water-dispersible granules (WG) (TT), 40% pyrimethanil suspension concentrate (SC) (PY), and 50% iprodione SC (IP)] and evaluated their effects on YLS abundance and WBPH survival rate. Both YLS abundance and adult WBPH survival rate were significantly decreased upon feeding fungicide-treated rice plants, and exposure to 50% IP resulted in the strongest reduction. The abundance of two Sf-YLS species (Ascomycetes symbiotes and Cla-like symbiotes) was significantly reduced upon exposure to 50% IP. The counts of Ascomycetes symbiotes, the most abundant YLS species, were also suppressed by the other fungicides tested. In conclusion, 50% IP was the most effective fungicide, reducing YLS abundance and WBPH survival rate under controlled conditions, suggesting its potential use to control WBPH.
KW - Ascomycetes symbiotes
KW - DGGE
KW - Effective strategy
KW - Synergistic effects
KW - YLS diversity
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U2 - 10.3390/insects11040209
DO - 10.3390/insects11040209
M3 - Article
C2 - 32230918
AN - SCOPUS:85082942109
SN - 2075-4450
VL - 11
JO - Insects
JF - Insects
IS - 4
M1 - 209
ER -