Fungicidal properties, sterol binding, and proteolytic resistance of the synthetic peptide D4E1

A. J. De Lucca; J. M. Bland; C. Grimm; T. J. Jacks; J. W. Cary; J. M. Jaynes; T. E. Cleveland; T. J. Walsh

doi:10.1139/w98-032

Fungicidal properties, sterol binding, and proteolytic resistance of the synthetic peptide D4E1

A. J. De Lucca, J. M. Bland, C. Grimm, T. J. Jacks, J. W. Cary, J. M. Jaynes, T. E. Cleveland, T. J. Walsh

School of Medicine

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45 Scopus citations

Abstract

The fungicidal properties of the synthetic peptide D4E1 were studied with nongerminated and germinating conidia of Aspergillus flavus, Aspergillus fumigatus, Aspergillus niger, Fusarium moniliforme, and Fusarium oxysporum. The minimal lethal concentrations (MLC) needed to kill 100% of germinating conidia of A. fumigatus, A. flavus, and A. niger were 12.5, 12.5, and 25 μM, respectively. The MLC value for nongerminated and germinating conidia of both Fusarium spp. was 3.0 μM. Except for A. fumigatus, D4E1 was inactive against the nongerminated conidia of the Aspergillus spp. Physicochemical studies showed D4E1 complexed with ergosterol, a sterol present in conidial walls. Cholesterol, present in nongerminated conidia of F. moniliforme, had a greater affinity for D4E1 than did ergosterol. D4E1 was more resistant to fungal and plant protease degradation than the natural peptide, cecropin A. These in vitro results suggest D4E1 is a candidate for transgenic expression in plants to enhance host resistance to fungal infection.

Original language	English (US)
Pages (from-to)	514-520
Number of pages	7
Journal	Canadian Journal of Microbiology
Volume	44
Issue number	6
DOIs	https://doi.org/10.1139/w98-032
State	Published - Jan 1 1998

Keywords

Aspergillus spp.
Fungicidal
Fusarium spp.
Peptides
Sterols

ASJC Scopus subject areas

Microbiology
Immunology
Applied Microbiology and Biotechnology
Molecular Biology
Genetics

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title = "Fungicidal properties, sterol binding, and proteolytic resistance of the synthetic peptide D4E1",

abstract = "The fungicidal properties of the synthetic peptide D4E1 were studied with nongerminated and germinating conidia of Aspergillus flavus, Aspergillus fumigatus, Aspergillus niger, Fusarium moniliforme, and Fusarium oxysporum. The minimal lethal concentrations (MLC) needed to kill 100% of germinating conidia of A. fumigatus, A. flavus, and A. niger were 12.5, 12.5, and 25 μM, respectively. The MLC value for nongerminated and germinating conidia of both Fusarium spp. was 3.0 μM. Except for A. fumigatus, D4E1 was inactive against the nongerminated conidia of the Aspergillus spp. Physicochemical studies showed D4E1 complexed with ergosterol, a sterol present in conidial walls. Cholesterol, present in nongerminated conidia of F. moniliforme, had a greater affinity for D4E1 than did ergosterol. D4E1 was more resistant to fungal and plant protease degradation than the natural peptide, cecropin A. These in vitro results suggest D4E1 is a candidate for transgenic expression in plants to enhance host resistance to fungal infection.",

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AU - De Lucca, A. J.

AU - Bland, J. M.

AU - Grimm, C.

AU - Jacks, T. J.

AU - Cary, J. W.

AU - Jaynes, J. M.

AU - Cleveland, T. E.

AU - Walsh, T. J.

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Fungicidal properties, sterol binding, and proteolytic resistance of the synthetic peptide D4E1

Abstract

Keywords

ASJC Scopus subject areas

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