Fungicidal effect of pleurocidin by membrane-active mechanism and design of enantiomeric analogue for proteolytic resistance

Hyun Jun Jung, Yoonkyung Park, Woo Sang Sung, Bo Kyoung Suh, Juneyoung Lee, Kyung Soo Hahm, Dong Gun Lee

Research output: Contribution to journalArticle

Abstract

Pleurocidin (Ple) is a 25-residue peptide which is derived from the skin mucous secretion of the winter flounder (Pleuronectes americanus). In this study, we investigated antifungal effects and its mode of action of Ple on human pathogenic fungi. Ple showed potent antifungal activity with low hemolytic activity. To investigate the antifungal mechanisms of Ple, the cellular localization and membrane interaction of Ple were examined. Protoplast regeneration and membrane-disrupting activity by DPH-labeled membrane support the idea, that Ple exerts fungicidal activity against the human pathogenic fungus Candida albicans with the disruption of a plasma membrane. To aim for which was the application of a therapeutic agent, we designed a synthetic enantiomeric peptide composed of all-d-amino acids to enhance proteolytic resistance. The synthetic all-d-Ple also displayed two-fold more potent antifungal activity than that of all-l-Ple, and its antifungal activity showed proteolytic resistance against various proteases. Therefore, these results suggest a therapeutic potential of all-d-Ple with regard to its proteolytic resistance against human fungal infections.

Original languageEnglish (US)
Pages (from-to)1400-1405
Number of pages6
JournalBiochimica et Biophysica Acta - Biomembranes
Volume1768
Issue number6
DOIs
StatePublished - Jun 1 2007
Externally publishedYes

Keywords

  • Antimicrobial peptide
  • Fungicidal activity
  • Membrane disrupting activity
  • Pleurocidin
  • Protease resistance

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Cell Biology

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