Fungicidal and binding properties of the natural peptides cecropin B and dermaseptin

A. J. De Lucca, J. M. Bland, T. J. Jacks, C. Grimm, T. J. Walsh

Research output: Contribution to journalArticle

Abstract

In vitro fungicidal properties of cecropin B and dermaseptin were explored using non-germinating and germinating conidia from Aspergillus flavus, A. fumigatus, A. niger, Fusarium moniliforme and F. oxysporum. Cecropin B produced LD50 values for germinating A. flavus, A. fumigatus and A. niger conidia of 3.0, 0.5 and 2.0 μM, respectively, while dermaseptin gave LD50 values of 4.0, 0.05 and 2.0 μM respectively. Cecropin B produced an LD50 value of 0.2 μM for non-germinating F. moniliforme and F. oxysporum conidia, while dermaseptin did not reduce either as much as 50% at any level tested. LD50 levels for CB were 0.2 and 0.1 μM, respectively, for germinating F. moniliforme and F. oxysporum conidia. Dermaseptin was less effective, giving LD50 values for germinating F. moniliforme and F. oxysporum conidia of 0.3 and 0.8 μM, respectively. Neither peptide reduced conidial viabilities of non-germinating Aspergillus spp. Physicochemical studies indicated cecropin B and dermaseptin bound to ergosterol and cholesterol, conidial wall constituents, but not to chitin or β-1,3-glucan.

Original languageEnglish (US)
Pages (from-to)291-298
Number of pages8
JournalMedical Mycology
Volume36
Issue number5
DOIs
StatePublished - Oct 1998
Externally publishedYes

Fingerprint

antifungal properties
Fungal Spores
binding properties
Lethal Dose 50
lethal dose 50
conidia
peptides
Aspergillus flavus
Aspergillus fumigatus
Ergosterol
Chitin
Glucans
ergosterol
Fusarium
glucans
Aspergillus
chitin
Fusarium oxysporum
Aspergillus niger
Cholesterol

Keywords

  • Cecropin B
  • Conidia
  • Dermaseptin
  • Fungicidal
  • Peptides

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • veterinary(all)
  • Microbiology

Cite this

De Lucca, A. J., Bland, J. M., Jacks, T. J., Grimm, C., & Walsh, T. J. (1998). Fungicidal and binding properties of the natural peptides cecropin B and dermaseptin. Medical Mycology, 36(5), 291-298. https://doi.org/10.1046/j.1365-280X.1998.00160.x

Fungicidal and binding properties of the natural peptides cecropin B and dermaseptin. / De Lucca, A. J.; Bland, J. M.; Jacks, T. J.; Grimm, C.; Walsh, T. J.

In: Medical Mycology, Vol. 36, No. 5, 10.1998, p. 291-298.

Research output: Contribution to journalArticle

De Lucca, A. J. ; Bland, J. M. ; Jacks, T. J. ; Grimm, C. ; Walsh, T. J. / Fungicidal and binding properties of the natural peptides cecropin B and dermaseptin. In: Medical Mycology. 1998 ; Vol. 36, No. 5. pp. 291-298.
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