Fundamentals of Light Microscopy and Electronic Imaging: Second Edition

Douglas B. Murphy, Michael W. Davidson

Research output: Book/ReportBook

Abstract

Fundamentals of Light Microscopy and Electronic Imaging, Second Edition provides a coherent introduction to the principles and applications of the integrated optical microscope system, covering both theoretical and practical considerations. It expands and updates discussions of multi-spectral imaging, intensified digital cameras, signal colocalization, and uses of objectives, and offers guidance in the selection of microscopes and electronic cameras, as well as appropriate auxiliary optical systems and fluorescent tags. The book is divided into three sections covering optical principles in diffraction and image formation, basic modes of light microscopy, and components of modern electronic imaging systems and image processing operations. Each chapter introduces relevant theory, followed by descriptions of instrument alignment and image interpretation. This revision includes new chapters on live cell imaging, measurement of protein dynamics, deconvolution microscopy, and interference microscopy.

Original languageEnglish (US)
PublisherJohn Wiley and Sons
ISBN (Print)9780471692140
DOIs
StatePublished - Sep 13 2012

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ASJC Scopus subject areas

  • Physics and Astronomy(all)
  • Medicine(all)

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