Functional roles of mannose-binding protein in the adhesion, cytotoxicity and phagocytosis of Acanthamoeba castellanii

Jong Hyun Kim, Abdul Matin, Ho Joon Shin, Hyun Park, Kyung Tae Yoo, Xi Zhe Yuan, Kwang Sik Kim, Suk Yul Jung

Research output: Contribution to journalArticle

Abstract

Acanthamoeba castellanii is a single-celled protozoan that is widely distributed in the environment and is a well-known of causing human keratitis, a vision-threatening infection. In this study, an ethyl methane sulfonate (EMS) and a selection of saccharide were applied to A. castellanii by chemical mutagenesis. To understand the functional roles of a mannose-binding protein (MBP). A. castellanii were treated with methyl-alpha- d-mannopyranoside abbreviated Man, with and without the EMS pre-treatment, and their adhesion and cytotoxicity were analyzed, using a human brain microvascular endothelial cell (HBMEC) as the target cell. Both EMS and Man mutants exhibited significantly decreased levels of MBP expression and cytotoxicity to HBMEC, but showed similar levels of binding to HBMEC, as compared with the wild type. Of interest was that the exogenous mannose inhibited amoebae (i.e., Man mutant) binding to the HBMEC by

Original languageEnglish (US)
Pages (from-to)287-292
Number of pages6
JournalExperimental Parasitology
Volume132
Issue number2
DOIs
StatePublished - Oct 2012

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Acanthamoeba castellanii
Mannose-Binding Lectin
Phagocytosis
Methane
Endothelial Cells
Brain
Mannose
Amoeba
Keratitis
Mutagenesis
Infection

Keywords

  • Acanthamoeba castellanii
  • Ethyl methane sulfonate
  • Mannose binding protein

ASJC Scopus subject areas

  • Parasitology
  • Immunology

Cite this

Functional roles of mannose-binding protein in the adhesion, cytotoxicity and phagocytosis of Acanthamoeba castellanii. / Kim, Jong Hyun; Matin, Abdul; Shin, Ho Joon; Park, Hyun; Yoo, Kyung Tae; Yuan, Xi Zhe; Kim, Kwang Sik; Jung, Suk Yul.

In: Experimental Parasitology, Vol. 132, No. 2, 10.2012, p. 287-292.

Research output: Contribution to journalArticle

Kim, Jong Hyun ; Matin, Abdul ; Shin, Ho Joon ; Park, Hyun ; Yoo, Kyung Tae ; Yuan, Xi Zhe ; Kim, Kwang Sik ; Jung, Suk Yul. / Functional roles of mannose-binding protein in the adhesion, cytotoxicity and phagocytosis of Acanthamoeba castellanii. In: Experimental Parasitology. 2012 ; Vol. 132, No. 2. pp. 287-292.
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