Functional role of laminin α1 chain during cerebellum development

Céline Heng, Olivier Lefebvre, Annick Klein, Malia M. Edwards, Patricia Simon-Assmann, Gertraud Orend, Dominique Bagnard

Research output: Contribution to journalArticle

Abstract

We had developed a conditional Laminin α1 knockout-mouse model (Lama1cko) bypassing embryonic lethality of Lama1 deficient mice to study the role of this crucial laminin chain during late developmental phases and organogenesis. Here, we report a strong defect in the organization of the adult cerebellum of Lama1cko mice. Our study of the postnatal cerebellum of Lama1cko animals revealed a disrupted basement membrane correlated with an unexpected excessive proliferation of granule cell precursors in the external granular layer (EGL). This was counteracted by a massive cell death occurring between the postnatal day 7 (P7) and day 20 (P20) resulting in a net balance of less cells and a smaller cerebellum. Our data show that the absence of Lama1 has an impact on the Bergmann glia scaffold that aberrantly develops. This phenotype is presumably responsible for the observed misplacing of granule cells that may explain the overall perturbation of the layering of the cerebellum and an aberrant folia formation.

Original languageEnglish (US)
Pages (from-to)480-489
Number of pages10
JournalCell Adhesion and Migration
Volume5
Issue number6
DOIs
StatePublished - Jan 1 2011

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Keywords

  • Cell migration
  • Cerebellum
  • Development
  • Laminin
  • Laminin-111

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience
  • Cell Biology

Cite this

Heng, C., Lefebvre, O., Klein, A., Edwards, M. M., Simon-Assmann, P., Orend, G., & Bagnard, D. (2011). Functional role of laminin α1 chain during cerebellum development. Cell Adhesion and Migration, 5(6), 480-489. https://doi.org/10.4161/cam.5.6.19191