Functional requirement of aquaporin-5 in plasma membranes of sweat glands

Lene N. Nejsum, Tae Hwan Kwon, Uffe B. Jensen, Ornella Fumagalli, Jørgen Frøkiaer, Carissa M. Krane, Anil G. Menon, Landon Stuart King, Peter C Agre, Søren Nielsen

Research output: Contribution to journalArticle

Abstract

The distribution and function of aquaporins (AQPs) have not previously been defined in sweat glands. In this study, AQP1, AQP3, and AQP5 mRNA were demonstrated in rat paw by reverse transcription (RT)-PCR, but AQP2 and AQP4 were not. AQP1, AQP3, and AQP5 protein were confirmed in these tissues by immunoblotting. AQP1 was identified in capillary endothelial cells by immunohistochemical labeling, but not in sweat glands or epidermis. Abundant AQP3 expression was seen in basal levels of epidermis, but not in sweat glands. AQP2 and AQP4 were not observed in either skin or sweat glands. Immunohistochemical labeling revealed abundant AQP5 in secretory parts of rat and mouse sweat glands, where immunoelectron microscopy demonstrated abundant AQP5 labeling in the apical plasma membrane. AQP5 immunolabeling of human sweat glands yielded a similar pattern. To establish the role of AQP5 in sweat secretion, we tested the response of adult mice to s.c. injection of pilocarpine, as visualized by reaction of secreted amylase with iodine/starch. The number of active sweat glands was dramatically reduced in AQP5-null (-/-) mice compared with heterozygous (+/-) and wild-type (+/+) mice. We conclude that the presence of AQP5 in plasma membranes of sweat glands is essential for secretion, providing potential insight into mechanisms underlying mammalian thermo-regulation, tactile sensitivity, and the pathophysiology of hyperhidrosis.

Original languageEnglish (US)
Pages (from-to)511-516
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume99
Issue number1
DOIs
StatePublished - Jan 8 2002
Externally publishedYes

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Aquaporin 5
Sweat Glands
Cell Membrane
Epidermis
Aquaporin 3
Aquaporin 1
Hyperhidrosis
Aquaporins
Pilocarpine
Immunoelectron Microscopy
Sweat
Touch
Amylases
Immunoblotting
Iodine
Starch
Reverse Transcription
Endothelial Cells

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Nejsum, L. N., Kwon, T. H., Jensen, U. B., Fumagalli, O., Frøkiaer, J., Krane, C. M., ... Nielsen, S. (2002). Functional requirement of aquaporin-5 in plasma membranes of sweat glands. Proceedings of the National Academy of Sciences of the United States of America, 99(1), 511-516. https://doi.org/10.1073/pnas.012588099

Functional requirement of aquaporin-5 in plasma membranes of sweat glands. / Nejsum, Lene N.; Kwon, Tae Hwan; Jensen, Uffe B.; Fumagalli, Ornella; Frøkiaer, Jørgen; Krane, Carissa M.; Menon, Anil G.; King, Landon Stuart; Agre, Peter C; Nielsen, Søren.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, No. 1, 08.01.2002, p. 511-516.

Research output: Contribution to journalArticle

Nejsum, Lene N. ; Kwon, Tae Hwan ; Jensen, Uffe B. ; Fumagalli, Ornella ; Frøkiaer, Jørgen ; Krane, Carissa M. ; Menon, Anil G. ; King, Landon Stuart ; Agre, Peter C ; Nielsen, Søren. / Functional requirement of aquaporin-5 in plasma membranes of sweat glands. In: Proceedings of the National Academy of Sciences of the United States of America. 2002 ; Vol. 99, No. 1. pp. 511-516.
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