Functional reconstitution and characterization of AqpZ, the E. coli water channel protein

Mario J. Borgnia, David Kozono, Giuseppe Calamita, Peter C. Maloney, Peter Agre

Research output: Contribution to journalArticlepeer-review

Abstract

Understanding the selectivity of aquaporin water channels will require structural and functional studies of wild-type and modified proteins; however, expression systems have not previously yielded aquaporins in the necessary milligram quantities. Here we report expression of a histidine-tagged form of Escherichia coli aquaporin-Z (AqpZ) in its homologous expression system. 10-His-AqpZ is solubilized and purified to near homogeneity in a single step with a final yield of ~2.5 mg/l of culture. The histidine tag is removed by trypsin, yielding the native protein with the addition of three N-terminal residues, as confirmed by microsequencing. Sucrose gradient sedimentation analysis showed that the native, solubilized AqpZ protein is a trypsin-resistant tetramer. Unlike other known aquaporins, AqpZ tetramers are not readily dissociated by 1% SDS at neutral pH. Hydrophilic reducing agents have a limited effect on the stability of the tetramer in 1% SDS, whereas incubations for more than 24 hours, pH values below 5.6, or exposure to the hydrophobic reducing agent ethanedithiol cause dissociation into monomers. Cys20, but not Cys9, is necessary for the stability of the AqpZ tetramer in SDS. Upon reconstitution into proteoliposomes, AqpZ displays very high osmotic water permeability (p(f) ≥ 10 x 10-14 cm3 s-1 subunit-1) and low Arrhenius activation energy (E(a) = 3.7 kcal/mol), similar to mammalian aquaporin-1 (AQP1). No permeation by glycerol, urea or sorbitol was detected. Expression of native and modified AqpZ in milligram quantities has permitted biophysical characterization of this remarkably stable aquaporin tetramer, which is being utilized for high-resolution structural studies.

Original languageEnglish (US)
Pages (from-to)1169-1179
Number of pages11
JournalJournal of molecular biology
Volume291
Issue number5
DOIs
StatePublished - Sep 3 1999

Keywords

  • Aquaporin
  • Bacterial
  • Channel structure
  • Gene family
  • Water transport

ASJC Scopus subject areas

  • Structural Biology
  • Molecular Biology

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