Functional neuroanatomy of CCK4-induced anxiety in normal healthy volunteers

C. Benkelfat, J. Bradwejn, E. Meyer, M. Ellenbogen, S. Milot, A. Gjedde, A. Evans

Research output: Contribution to journalArticle

Abstract

Objective: The authors tested the prediction of temporal cortex activation during experimentally induced anxiety by using positron emission tomography and the [15O]H2O bolus-subtraction method to determine regional cerebral blood flow (CBF) changes in normal volunteers challenged with a bolus injection of cholecystokinin tetrapeptide (CCK4). Method: Eight right- handed healthy subjects (five male, three female; mean age, 26.4 years) underwent four 60-second [15O]H2O scans separated by 15-minute intervals; each scan followed an intravenous bolus injection of either saline (placebo) or CCK4 (50 μg). Each subject received CCK4 once, as the first or second bolus, in a random-order, placebo-controlled, double-blind fashion. Two of the three placebo conditions were nominally identical, and the remaining placebo was used to control for anticipatory anxiety. Magnetic resonance imaging scans were obtained for subsequent anatomical correlation of blood flow changes. Results: CCK4, but not placebo, elicited a marked anxiogenic response, reflected by robust increases in subjective anxiety ratings and heart rate. CCK4-induced anxiety was associated with 1) robust and bilateral increases in extracerebral blood flow in the vicinity of the superficial temporal artery territory and 2) CBF increases in the anterior cingulate gyrus, the claustrum-insular-amygdala region, and the cerebellar vermis. Conclusions: Some of the temporopolar cortex CBF activation peaks previously reported in humans in association with drug- and non-drug-induced anxiety, as well as the increase in regional CBF in the claustrum-insular-amygdala region, may be of vascular and/or muscular origin.

Original languageEnglish (US)
Pages (from-to)1180-1184
Number of pages5
JournalAmerican Journal of Psychiatry
Volume152
Issue number8
StatePublished - Aug 1995
Externally publishedYes

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Cerebrovascular Circulation
Neuroanatomy
Healthy Volunteers
Anxiety
Placebos
Regional Blood Flow
Amygdala
Basal Ganglia
Tetragastrin
Temporal Arteries
Gyrus Cinguli
Temporal Lobe
Intravenous Injections
Positron-Emission Tomography
Blood Vessels
Heart Rate
Magnetic Resonance Imaging
Injections
Pharmaceutical Preparations

ASJC Scopus subject areas

  • Psychiatry and Mental health

Cite this

Benkelfat, C., Bradwejn, J., Meyer, E., Ellenbogen, M., Milot, S., Gjedde, A., & Evans, A. (1995). Functional neuroanatomy of CCK4-induced anxiety in normal healthy volunteers. American Journal of Psychiatry, 152(8), 1180-1184.

Functional neuroanatomy of CCK4-induced anxiety in normal healthy volunteers. / Benkelfat, C.; Bradwejn, J.; Meyer, E.; Ellenbogen, M.; Milot, S.; Gjedde, A.; Evans, A.

In: American Journal of Psychiatry, Vol. 152, No. 8, 08.1995, p. 1180-1184.

Research output: Contribution to journalArticle

Benkelfat, C, Bradwejn, J, Meyer, E, Ellenbogen, M, Milot, S, Gjedde, A & Evans, A 1995, 'Functional neuroanatomy of CCK4-induced anxiety in normal healthy volunteers', American Journal of Psychiatry, vol. 152, no. 8, pp. 1180-1184.
Benkelfat C, Bradwejn J, Meyer E, Ellenbogen M, Milot S, Gjedde A et al. Functional neuroanatomy of CCK4-induced anxiety in normal healthy volunteers. American Journal of Psychiatry. 1995 Aug;152(8):1180-1184.
Benkelfat, C. ; Bradwejn, J. ; Meyer, E. ; Ellenbogen, M. ; Milot, S. ; Gjedde, A. ; Evans, A. / Functional neuroanatomy of CCK4-induced anxiety in normal healthy volunteers. In: American Journal of Psychiatry. 1995 ; Vol. 152, No. 8. pp. 1180-1184.
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