Abstract
The instability of the fushi tarazu (ftz) mRNA is essential for the proper development of the Drosophila embryo. Previously, we identified a 201-nucleotide instability element (FIE3) in the 3′ untranslated region (UTR) of the ftz mRNA. Here we report on the identification of two additional elements in the protein-coding region of the message: the 63-nucleotide-long FIE5-1 and the 69-nucleotide-long FIE5-2. The function of both elements was position-dependent; the same elements destabilized RNAs when present within the coding region but did not when embedded in the 3′ UTR of the hybrid mRNAs. We conclude that ftz mRNA has three redundant instability elements, two in the protein-coding region and one in the 3′ UTR. Although each instability element is sufficient to destabilize a heterologous mRNA, the destabilizing activity of the two 5′-elements depended on their position within the message.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 23525-23530 |
| Number of pages | 6 |
| Journal | Journal of Biological Chemistry |
| Volume | 276 |
| Issue number | 26 |
| DOIs | |
| State | Published - Jun 29 2001 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Biochemistry
Cite this
Functional Mapping of Destabilizing Elements in the Protein-coding Region of the Drosophila fushi tarazu mRNA. / Ito, Jun Itsu; Jacobs-Lorena, Marcelo.
In: Journal of Biological Chemistry, Vol. 276, No. 26, 29.06.2001, p. 23525-23530.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Functional Mapping of Destabilizing Elements in the Protein-coding Region of the Drosophila fushi tarazu mRNA
AU - Ito, Jun Itsu
AU - Jacobs-Lorena, Marcelo
PY - 2001/6/29
Y1 - 2001/6/29
N2 - The instability of the fushi tarazu (ftz) mRNA is essential for the proper development of the Drosophila embryo. Previously, we identified a 201-nucleotide instability element (FIE3) in the 3′ untranslated region (UTR) of the ftz mRNA. Here we report on the identification of two additional elements in the protein-coding region of the message: the 63-nucleotide-long FIE5-1 and the 69-nucleotide-long FIE5-2. The function of both elements was position-dependent; the same elements destabilized RNAs when present within the coding region but did not when embedded in the 3′ UTR of the hybrid mRNAs. We conclude that ftz mRNA has three redundant instability elements, two in the protein-coding region and one in the 3′ UTR. Although each instability element is sufficient to destabilize a heterologous mRNA, the destabilizing activity of the two 5′-elements depended on their position within the message.
AB - The instability of the fushi tarazu (ftz) mRNA is essential for the proper development of the Drosophila embryo. Previously, we identified a 201-nucleotide instability element (FIE3) in the 3′ untranslated region (UTR) of the ftz mRNA. Here we report on the identification of two additional elements in the protein-coding region of the message: the 63-nucleotide-long FIE5-1 and the 69-nucleotide-long FIE5-2. The function of both elements was position-dependent; the same elements destabilized RNAs when present within the coding region but did not when embedded in the 3′ UTR of the hybrid mRNAs. We conclude that ftz mRNA has three redundant instability elements, two in the protein-coding region and one in the 3′ UTR. Although each instability element is sufficient to destabilize a heterologous mRNA, the destabilizing activity of the two 5′-elements depended on their position within the message.
UR - http://www.scopus.com/inward/record.url?scp=0035968285&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0035968285&partnerID=8YFLogxK
U2 - 10.1074/jbc.M102965200
DO - 10.1074/jbc.M102965200
M3 - Article
C2 - 11306592
AN - SCOPUS:0035968285
VL - 276
SP - 23525
EP - 23530
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
SN - 0021-9258
IS - 26
ER -