TY - JOUR
T1 - Functional heterogeneity of mutant rhodopsins responsible for autosomal dominant retinitis pigmentosa
AU - Sung, Ching Hwa
AU - Schneider, Barbara G.
AU - Agarwal, Neeraj
AU - Papermaster, David S.
AU - Nathans, Jeremy
PY - 1991/10/1
Y1 - 1991/10/1
N2 - Thirteen mutant rhodopsins responsible for autosomal dominant retinitis pigmentosa (ADRP) have been produced by transfection of cloned cDNA into tissue culture cells. Three mutants [class I: Phe-45 → Leu, Gin-344 → termination (deletion of C-terminal positions 344-348), and Pro-347 → Leu] resemble wild-type rhodopsin in yield, regenerability with 11-cis-retinal, and plasma membrane localization. Ten mutants [class II: Thr-17 → Met, Pro-23 → His, Thr-58 → Arg, Val-87 → Asp, Gly-89 → Asp, Gly-106 → Trp, Arg-135 → Leu, Arg-135 → Trp, Tyr-178 → Cys, and Asp-190 → Gly] accumulate to significantly lower levels, regenerate with 11-cis-retinal variably or not at all, and are transported inefficiently to the plasma membrane, remaining primarily in the endoplasmic reticulum. These data suggest that there are at least two distinct biochemical defects associated with different rhodopsin mutants in ADRP.
AB - Thirteen mutant rhodopsins responsible for autosomal dominant retinitis pigmentosa (ADRP) have been produced by transfection of cloned cDNA into tissue culture cells. Three mutants [class I: Phe-45 → Leu, Gin-344 → termination (deletion of C-terminal positions 344-348), and Pro-347 → Leu] resemble wild-type rhodopsin in yield, regenerability with 11-cis-retinal, and plasma membrane localization. Ten mutants [class II: Thr-17 → Met, Pro-23 → His, Thr-58 → Arg, Val-87 → Asp, Gly-89 → Asp, Gly-106 → Trp, Arg-135 → Leu, Arg-135 → Trp, Tyr-178 → Cys, and Asp-190 → Gly] accumulate to significantly lower levels, regenerate with 11-cis-retinal variably or not at all, and are transported inefficiently to the plasma membrane, remaining primarily in the endoplasmic reticulum. These data suggest that there are at least two distinct biochemical defects associated with different rhodopsin mutants in ADRP.
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M3 - Article
C2 - 1924344
AN - SCOPUS:0026058548
SN - 0027-8424
VL - 88
SP - 8840
EP - 8844
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 19
ER -