TY - JOUR
T1 - Functional expression in yeast of the human secretory pathway Ca2+, Mn2+-ATPase defective in Hailey-Hailey disease
AU - Ton, Van Khue
AU - Mandal, Debjani
AU - Vahadji, Cordelia
AU - Rao, Rajini
PY - 2002/2/22
Y1 - 2002/2/22
N2 - The discovery and biochemical characterization of the secretory pathway Ca2+-ATPase, PMR1, in Saccharomyces cerevisiae, has paved the way for identification of PMR1 homologues in many species including rat, Caenorhabditis elegans, and Homo sapiens. In yeast, PMR1 has been shown to function as a high affinity Ca2+/Mn2+ pump and has been localized to the Golgi compartment where it is important for protein sorting, processing, and glycosylation. However, little is known about PMR1 homologues in higher organisms. Loss of one functional allele of the human gene, hSPCA1, has been linked to Hailey-Hailey disease, characterized by skin ulceration and improper keratinocyte adhesion. We demonstrate that expression of hSPCA1 in yeast fully complements pmr1 phenotypes of hypersensitivity to Ca2+ chelators and Mn2+ toxicity. Similar to PMR1, epitope-tagged hSPCA1 also resides in the Golgi when expressed in yeast or in chinese hamster ovary cells. 45Ca2+ transport by hSPCA1 into isolated yeast Golgi vesicles shows an apparent Ca2+ affinity of 0.26 μM, is inhibitable by Mn2+, but is thapsigargin-insensitive. In contrast, heterologous expression of vertebrate sarcoplasmic reticulum and plasma membrane Ca2+-ATPases in yeast complement the Ca2+ - but not Mn2+-related phenotypes of the pmr1-null strain, suggesting that high affinity Mn2+ transport is a unique feature of the secretory pathway Ca2+-ATPases.
AB - The discovery and biochemical characterization of the secretory pathway Ca2+-ATPase, PMR1, in Saccharomyces cerevisiae, has paved the way for identification of PMR1 homologues in many species including rat, Caenorhabditis elegans, and Homo sapiens. In yeast, PMR1 has been shown to function as a high affinity Ca2+/Mn2+ pump and has been localized to the Golgi compartment where it is important for protein sorting, processing, and glycosylation. However, little is known about PMR1 homologues in higher organisms. Loss of one functional allele of the human gene, hSPCA1, has been linked to Hailey-Hailey disease, characterized by skin ulceration and improper keratinocyte adhesion. We demonstrate that expression of hSPCA1 in yeast fully complements pmr1 phenotypes of hypersensitivity to Ca2+ chelators and Mn2+ toxicity. Similar to PMR1, epitope-tagged hSPCA1 also resides in the Golgi when expressed in yeast or in chinese hamster ovary cells. 45Ca2+ transport by hSPCA1 into isolated yeast Golgi vesicles shows an apparent Ca2+ affinity of 0.26 μM, is inhibitable by Mn2+, but is thapsigargin-insensitive. In contrast, heterologous expression of vertebrate sarcoplasmic reticulum and plasma membrane Ca2+-ATPases in yeast complement the Ca2+ - but not Mn2+-related phenotypes of the pmr1-null strain, suggesting that high affinity Mn2+ transport is a unique feature of the secretory pathway Ca2+-ATPases.
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U2 - 10.1074/jbc.M110612200
DO - 10.1074/jbc.M110612200
M3 - Article
C2 - 11741891
AN - SCOPUS:0037155166
SN - 0021-9258
VL - 277
SP - 6422
EP - 6427
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
IS - 8
ER -