Functional expression and properties of a nicotinic α9/5-HT3 A chimeric receptor

Miguel Verbitsky, Paola V. Plazas, A. Belén Elgoyhen

Research output: Contribution to journalArticle

Abstract

We cescribe the functional properties of a nicotinic α9/serotonin subtype 3A (5HT3A) chimeric receptor expressed in Xenopus laevis oocytes. The chimera preserved ligand-binding properties of α9 and channel properties of 5HT3A.Thus, it responded to acetylcholine in a concentration-dependent manner with an EC50 of 70 μM but not to serotonin. It was blocked by methyllycaconitine, strychnine, atropine and nicotine, with the same rank order of potency as α9 receptors. The current-voltage relationship of currents through the α9/5HT3A chimera was similar to that of the 5HT3A receptors. These results are an evidence of functional coupling between the ligand-binding and the channel domains of the chimeric receptor.

Original languageEnglish (US)
Pages (from-to)1931-1934
Number of pages4
JournalNeuroReport
Volume14
Issue number15
DOIs
StatePublished - Oct 27 2003
Externally publishedYes

Keywords

  • Hair cells
  • Ion channels
  • Nicotinic receptors
  • Serotonin receptors

ASJC Scopus subject areas

  • Neuroscience(all)

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